A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Methods for obtaining combinatorial and array-based data as a function of temperature are needed in the chemical and biological sciences. It is presently quite difficult to employ temperature as a variable using standard wellplate, formats simply because it is very inconvenient to keep each well at a distinct temperature. In microfluidics, however, the situation is very different due to the short length scales involved. In this article, it is shown how a simple linear temperature gradient can be generated across dozens of parallel microfluidic channels simultaneously. This result is exploited to rapidly obtain activation energies from catalytic reactions, melting point transitions from lipid membranes, and fluorescence quantum yield curves from semiconductor nanocrystal probes as a function of temperature. The methods developed here could quite easily be extended to protein crystallization, phase diagram measurements, chemical reaction optimization, or multivariable experiments.

Original languageEnglish (US)
Pages (from-to)4432-4435
Number of pages4
JournalJournal of the American Chemical Society
Volume124
Issue number16
DOIs
StatePublished - Apr 24 2002

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Thermal gradients
Temperature
Quantum yield
Membrane Lipids
Crystallization
Quantum Dots
Nanocrystals
Biological Science Disciplines
Phase diagrams
Melting point
Chemical reactions
Activation energy
Fluorescence
Freezing
Semiconductor materials
Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

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abstract = "Methods for obtaining combinatorial and array-based data as a function of temperature are needed in the chemical and biological sciences. It is presently quite difficult to employ temperature as a variable using standard wellplate, formats simply because it is very inconvenient to keep each well at a distinct temperature. In microfluidics, however, the situation is very different due to the short length scales involved. In this article, it is shown how a simple linear temperature gradient can be generated across dozens of parallel microfluidic channels simultaneously. This result is exploited to rapidly obtain activation energies from catalytic reactions, melting point transitions from lipid membranes, and fluorescence quantum yield curves from semiconductor nanocrystal probes as a function of temperature. The methods developed here could quite easily be extended to protein crystallization, phase diagram measurements, chemical reaction optimization, or multivariable experiments.",
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A microfluidic device with a linear temperature gradient for parallel and combinatorial measurements. / Mao, Hanbin; Yang, Tinglu; Cremer, Paul S.

In: Journal of the American Chemical Society, Vol. 124, No. 16, 24.04.2002, p. 4432-4435.

Research output: Contribution to journalArticle

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