A microfluidic model for organ-specific extravasation of circulating tumor cells

R. Riahi, Y. L. Yang, H. Kim, L. Jiang, Pak Kin Wong, Y. Zohar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are the principal vehicle for the spread of non-hematologic cancer disease from a primary tumor, involving extravasation of CTCs across blood vessel walls, to form secondary tumors in remote organs. Herein, a polydimethylsiloxane-based microfluidic system is developed and characterized for in vitro systematic studies of organ-specific extravasation of CTCs. The system recapitulates the two major aspects of the in vivo extravasation microenvironment: local signaling chemokine gradients in a vessel with an endothelial monolayer. The parameters controlling the locally stable chemokine gradients, flow rate, and initial chemokine concentration are investigated experimentally and numerically. The microchannel surface treatment effect on the confluency and adhesion of the endothelial monolayer under applied shear flow has also been characterized experimentally. Further, the conditions for driving a suspension of CTCs through the microfluidic system are discussed while simultaneously maintaining both the local chemokine gradients and the confluent endothelial monolayer. Finally, the microfluidic system is utilized to demonstrate extravasation of MDA-MB-231 cancer cells in the presence of CXCL12 chemokine gradients. Consistent with the hypothesis of organ-specific extravasation, control experiments are presented to substantiate the observation that the MDA-MB-231 cell migration is attributed to chemotaxis rather than a random process.

Original languageEnglish (US)
Article number24103
JournalBiomicrofluidics
Volume8
Issue number2
DOIs
StatePublished - Mar 1 2014

Fingerprint

Circulating Neoplastic Cells
Microfluidics
Chemokines
organs
Tumors
tumors
Cells
multiple docking adapters
Monolayers
Neoplasms
gradients
Chemokine CXCL12
Chemotaxis
cancer
Cell Movement
Blood Vessels
Suspensions
random processes
blood vessels
Blood vessels

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Riahi, R. ; Yang, Y. L. ; Kim, H. ; Jiang, L. ; Wong, Pak Kin ; Zohar, Y. / A microfluidic model for organ-specific extravasation of circulating tumor cells. In: Biomicrofluidics. 2014 ; Vol. 8, No. 2.
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A microfluidic model for organ-specific extravasation of circulating tumor cells. / Riahi, R.; Yang, Y. L.; Kim, H.; Jiang, L.; Wong, Pak Kin; Zohar, Y.

In: Biomicrofluidics, Vol. 8, No. 2, 24103, 01.03.2014.

Research output: Contribution to journalArticle

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