A moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells

Laure Teysset, Jane C. Burns, Hiroko Shike, Barbara L. Sullivan, Alain Bucheton, Christophe Terzian

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that gypsy ENV- like ORF3 mediates viral infectivity. We have produced in the 293GP/LNhsp701ucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by the gypsy ENV-like protein. We have shown by immunostaining that the gypsy envelope protein is produced in 293GP/LNhsp701ucL.3 cells and that vector particles collected from these cells can infect Drosophila cells. Our results provide direct evidence that the infectious property of gypsy is due to its ORF3 gene product.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalJournal of virology
Volume72
Issue number1
DOIs
StatePublished - Jan 1998

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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