A moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells

Laure Teysset, Jane C. Burns, Hiroko Shike, Barbara L. Sullivan, Alain Bucheton, Christophe Terzian

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The gypsy element of Drosophila melanogaster is the first retrovirus identified so far in invertebrates. Previous data suggest that gypsy ENV- like ORF3 mediates viral infectivity. We have produced in the 293GP/LNhsp701ucL.3 human cell line a Moloney murine leukemia virus-based retroviral vector pseudotyped by the gypsy ENV-like protein. We have shown by immunostaining that the gypsy envelope protein is produced in 293GP/LNhsp701ucL.3 cells and that vector particles collected from these cells can infect Drosophila cells. Our results provide direct evidence that the infectious property of gypsy is due to its ORF3 gene product.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalJournal of virology
Volume72
Issue number1
StatePublished - Jan 1 1998

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Murine leukemia virus
Insect Vectors
retroviral vectors
Roma
Moloney murine leukemia virus
Drosophila
insects
Retroviridae
human cell lines
cells
Drosophila melanogaster
Invertebrates
pathogenicity
proteins
invertebrates
Proteins
Cell Line
genes
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Teysset, Laure ; Burns, Jane C. ; Shike, Hiroko ; Sullivan, Barbara L. ; Bucheton, Alain ; Terzian, Christophe. / A moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells. In: Journal of virology. 1998 ; Vol. 72, No. 1. pp. 853-856.
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A moloney murine leukemia virus-based retroviral vector pseudotyped by the insect retroviral gypsy envelope can infect Drosophila cells. / Teysset, Laure; Burns, Jane C.; Shike, Hiroko; Sullivan, Barbara L.; Bucheton, Alain; Terzian, Christophe.

In: Journal of virology, Vol. 72, No. 1, 01.01.1998, p. 853-856.

Research output: Contribution to journalArticle

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