TY - GEN
T1 - A multimodal gold nanorod-locked nucleic acid approach for probing individual cell gene expressions in living tissues
AU - Wang, S.
AU - Riahi, R.
AU - Long, M.
AU - Zhang, D. D.
AU - Wong, P. K.
N1 - Publisher Copyright:
© 14CBMS.
PY - 2014
Y1 - 2014
N2 - A multimodal gold nanorod-locked nucleic acid (GNR-LNA) approach is developed for perturbing and monitoring the spatiotemporal gene expression dynamics at the single cell level. We demonstrated (1) LNA-based detection of mRNA expression in single living cells as well as lung and liver tissues, (2) plasmonic GNR-mediated photothermal ablation of mice lung tissues for perturbing gene expression and single cell ablation, and (3) monitoring the spatiotemporal gene expression dynamics of individual cells after photothermal ablation. Experimental results demonstrated the applicability and specificity of GNR-LNA probe for detecting individual cell mRNA expression, providing an effective approach for investigating complex tissue morphogenic and regeneration processes.
AB - A multimodal gold nanorod-locked nucleic acid (GNR-LNA) approach is developed for perturbing and monitoring the spatiotemporal gene expression dynamics at the single cell level. We demonstrated (1) LNA-based detection of mRNA expression in single living cells as well as lung and liver tissues, (2) plasmonic GNR-mediated photothermal ablation of mice lung tissues for perturbing gene expression and single cell ablation, and (3) monitoring the spatiotemporal gene expression dynamics of individual cells after photothermal ablation. Experimental results demonstrated the applicability and specificity of GNR-LNA probe for detecting individual cell mRNA expression, providing an effective approach for investigating complex tissue morphogenic and regeneration processes.
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M3 - Conference contribution
AN - SCOPUS:84941642621
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1116
EP - 1118
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -