A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection

Xu Yu, Yiqiu Xia, Yi Tang, Wen Long Zhang, Yin-Ting Yeh, Huaguang Lu, Siyang Zheng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rapid and simultaneous detection of multiple potential pathogens by portable devices can facilitate early diagnosis of infectious diseases, and allow for rapid and effective implementation of disease prevention and treatment measures. The development of a ZnO nanorod integrated microdevice as a multiplex immunofluorescence platform for highly sensitive and selective detection of avian influenza virus (AIV) is described. The 3D morphology and unique optical property of the ZnO nanorods boost the detection limit of the H5N2 AIV to as low as 3.6 × 10 3 EID 50 mL −1 (EID 50 : 50% embryo infectious dose), which is ≈22 times more sensitive than conventional enzyme-linked immunosorbent assay. The entire virus capture and detection process could be completed within 1.5 h with excellent selectivity. Moreover, this microfluidic biosensor is capable of detecting multiple viruses simultaneously by spatial encoding of capture antibodies. One prominent feature of the device is that the captured H5N2 AIV can be released by simply dissolving ZnO nanorods under slightly acidic environment for subsequent off-chip analyses. As a whole, this platform provides a powerful tool for rapid detection of multiple pathogens, which may extent to the other fields for low-cost and convenient biomarker detection.

Original languageEnglish (US)
Article number1700425
JournalSmall
Volume13
Issue number24
DOIs
StatePublished - Jun 27 2017

Fingerprint

Nanotubes
Influenza in Birds
Microfluidics
Pathogens
Orthomyxoviridae
Viruses
Immunoassay
Nanorods
Equipment and Supplies
Biosensing Techniques
Fluorescent Antibody Technique
Communicable Diseases
Limit of Detection
Early Diagnosis
Embryonic Structures
Biomarkers
Enzyme-Linked Immunosorbent Assay
Immunosorbents
Costs and Cost Analysis
Biosensors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Engineering (miscellaneous)

Cite this

Yu, Xu ; Xia, Yiqiu ; Tang, Yi ; Zhang, Wen Long ; Yeh, Yin-Ting ; Lu, Huaguang ; Zheng, Siyang. / A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection. In: Small. 2017 ; Vol. 13, No. 24.
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A Nanostructured Microfluidic Immunoassay Platform for Highly Sensitive Infectious Pathogen Detection. / Yu, Xu; Xia, Yiqiu; Tang, Yi; Zhang, Wen Long; Yeh, Yin-Ting; Lu, Huaguang; Zheng, Siyang.

In: Small, Vol. 13, No. 24, 1700425, 27.06.2017.

Research output: Contribution to journalArticle

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