A new biodegradable polyester elastomer for cartilage tissue engineering

Yong Kang, Jian Yang, Sadiya Khan, Lucas Anissian, Guillermo A. Ameer

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The objective of this study is to assess whether a new biodegradable elastomer, poly(1,8-octanediol citrate) (POC), would be a suitable material to engineer elastomeric scaffolds for cartilage tissue engineering. Porous POC scaffolds were prepared via the salt-leaching method and initially assessed for their ability to rapidly recover from compressive deformation (% recovery ratio). Controls consisted of scaffolds made from other materials commonly used in cartilage tissue engineering, including 2% agarose, 4% alginate, non woven poly(glycolic acid) (PGA) meshes, and non woven poly(L-lactide-co-glycolide) (PLGA) meshes. Articular chondrocytes from bovine knee were isolated and seeded onto porous disk-shaped POC scaffolds, which were subsequently cultured in vitro for up to 28 days. POC scaffolds completely recover from compressive deformation, and the stress-strain curve is typical of an elastomer (recovery ratio > 98%). Agarose gel (2%) scaffolds broke during the compression test. The recovery ratio of 4% alginate gel scaffolds, PLLA, and PGA were 72, 85, and 88%, respectively. The Young's modulus of POC-chondrocyte constructs and cell-free POC scaffolds cultured for 28 days were 12.02 ± 2.26 kPa and 3.27 ± 0.72 kPa, respectively. After 28 days of culture, the recovery ratio of POC-chondrocyte constructs and cell-free POC scaffolds were 93% and 99%, respectively. The glycosaminoglycan (GAG) and collagen content at day 28 was 36% and 26% of that found in bovine knee cartilage explants. Histology/immunohistochemistry evaluations confirm that chondrocytes were able to attach to the pore walls within the scaffold, maintain cell phenotype, and form a cartilaginous tissue during the 28 days of culture.

Original languageEnglish (US)
Pages (from-to)331-339
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume77
Issue number2
DOIs
StatePublished - May 1 2006

Fingerprint

Elastomers
Polyesters
Cartilage
Tissue engineering
Scaffolds
Prostaglandins A
Recovery
glycolic acid
Alginate
Scaffolds (biology)
Sepharose
Gels
Forms (concrete)
Histology
Stress-strain curves
Glycosaminoglycans
Collagen
Leaching
Salts
Elastic moduli

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Kang, Yong ; Yang, Jian ; Khan, Sadiya ; Anissian, Lucas ; Ameer, Guillermo A. / A new biodegradable polyester elastomer for cartilage tissue engineering. In: Journal of Biomedical Materials Research - Part A. 2006 ; Vol. 77, No. 2. pp. 331-339.
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abstract = "The objective of this study is to assess whether a new biodegradable elastomer, poly(1,8-octanediol citrate) (POC), would be a suitable material to engineer elastomeric scaffolds for cartilage tissue engineering. Porous POC scaffolds were prepared via the salt-leaching method and initially assessed for their ability to rapidly recover from compressive deformation ({\%} recovery ratio). Controls consisted of scaffolds made from other materials commonly used in cartilage tissue engineering, including 2{\%} agarose, 4{\%} alginate, non woven poly(glycolic acid) (PGA) meshes, and non woven poly(L-lactide-co-glycolide) (PLGA) meshes. Articular chondrocytes from bovine knee were isolated and seeded onto porous disk-shaped POC scaffolds, which were subsequently cultured in vitro for up to 28 days. POC scaffolds completely recover from compressive deformation, and the stress-strain curve is typical of an elastomer (recovery ratio > 98{\%}). Agarose gel (2{\%}) scaffolds broke during the compression test. The recovery ratio of 4{\%} alginate gel scaffolds, PLLA, and PGA were 72, 85, and 88{\%}, respectively. The Young's modulus of POC-chondrocyte constructs and cell-free POC scaffolds cultured for 28 days were 12.02 ± 2.26 kPa and 3.27 ± 0.72 kPa, respectively. After 28 days of culture, the recovery ratio of POC-chondrocyte constructs and cell-free POC scaffolds were 93{\%} and 99{\%}, respectively. The glycosaminoglycan (GAG) and collagen content at day 28 was 36{\%} and 26{\%} of that found in bovine knee cartilage explants. Histology/immunohistochemistry evaluations confirm that chondrocytes were able to attach to the pore walls within the scaffold, maintain cell phenotype, and form a cartilaginous tissue during the 28 days of culture.",
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A new biodegradable polyester elastomer for cartilage tissue engineering. / Kang, Yong; Yang, Jian; Khan, Sadiya; Anissian, Lucas; Ameer, Guillermo A.

In: Journal of Biomedical Materials Research - Part A, Vol. 77, No. 2, 01.05.2006, p. 331-339.

Research output: Contribution to journalArticle

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