A new dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions

Stephen B. Martin, Elizabeth S. Schauer, David H. Blum, Paul A. Kremer, William P. Bahnfleth, James Freihaut

Research output: Contribution to journalArticle

Abstract

We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k = 0.1471 cm2/mJ (with 95% confidence bounds of 0.1426 to 0.1516).

Original languageEnglish (US)
Pages (from-to)674-680
Number of pages7
JournalJournal of Photochemistry and Photobiology B: Biology
Volume162
DOIs
StatePublished - Sep 1 2016

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Computer Systems
collimation
microorganisms
Bacillus subtilis
Spores
deactivation
Suspensions
reactors
irradiance
spores
Bacillus
luminaires
confidence
fluence
Direction compound
dosage
energy

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

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abstract = "We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k = 0.1471 cm2/mJ (with 95{\%} confidence bounds of 0.1426 to 0.1516).",
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A new dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions. / Martin, Stephen B.; Schauer, Elizabeth S.; Blum, David H.; Kremer, Paul A.; Bahnfleth, William P.; Freihaut, James.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 162, 01.09.2016, p. 674-680.

Research output: Contribution to journalArticle

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