A new histogenetic method for minor histocompatibility antigen typing

V. Hauptfeld, J. Klein

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A new method for typing minor H antigens is described. The method is based on the observation that effector T cells, sensitized in vivo to minor H antigens and then rechallenged in vitro, will lyse labeled target cells in the CML assay, provided that the target, the sensitizing, and the responding cells share the same allele at H-2K or H-2D loci. In this method, two congenic lines differing at a minor H locus are cross-immunized (by skin grafting, injection of lymphoid cells, or, preferably, by a combination of both treatments), rechallenged in vitro, and tested against a panel of strains carrying the same H-2 haplotype as the two lines. Target cells of the panel carrying the same minor H antigens as the strain against which the effector cells were sensitized are lysed; target cells not carrying these antigens are unaffected. The feasibility of the method was demonstrated by testing for H-3 antigens of a panel of H-2b-bearing strains. The new method is faster and more economical than the classical F1 method which has been used until recently.

Original languageEnglish (US)
Pages (from-to)423-426
Number of pages4
JournalJournal of Immunology
Volume118
Issue number2
StatePublished - 1977

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Minor Histocompatibility Antigens
Skin Transplantation
Haplotypes
Alleles
Lymphocytes
T-Lymphocytes
Antigens
Injections
H antigen

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Hauptfeld, V. ; Klein, J. / A new histogenetic method for minor histocompatibility antigen typing. In: Journal of Immunology. 1977 ; Vol. 118, No. 2. pp. 423-426.
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Hauptfeld, V & Klein, J 1977, 'A new histogenetic method for minor histocompatibility antigen typing', Journal of Immunology, vol. 118, no. 2, pp. 423-426.

A new histogenetic method for minor histocompatibility antigen typing. / Hauptfeld, V.; Klein, J.

In: Journal of Immunology, Vol. 118, No. 2, 1977, p. 423-426.

Research output: Contribution to journalArticle

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AU - Klein, J.

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