A new model for nuclear envelope breakdown

M. Terasaki, P. Campagnola, M. M. Rolls, P. A. Stein, J. Ellenberg, B. Hinkle, B. Slepchenko

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Abstract

Nuclear envelope breakdown was investigated during meiotic maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as monitored by confocal microscopy, consists of two phases, a slow uniform increase and then a massive wave. From quantitative analysis of the first phase of dextran entry, and from imaging of green fluorescent protein chimeras, we conclude that nuclear pore disassembly begins several minutes before nuclear envelope breakdown. The best fit for the second phase of entry is with a spreading disruption of the membrane permeability barrier determined by three-dimensional computer simulations of diffusion. We propose a new model for the mechanism of nuclear envelope breakdown in which disassembly of the nuclear pores leads to a fenestration of the nuclear envelope double membrane.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalMolecular biology of the cell
Volume12
Issue number2
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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    Terasaki, M., Campagnola, P., Rolls, M. M., Stein, P. A., Ellenberg, J., Hinkle, B., & Slepchenko, B. (2001). A new model for nuclear envelope breakdown. Molecular biology of the cell, 12(2), 503-510. https://doi.org/10.1091/mbc.12.2.503