A new paper-based platform technology for point-of-care diagnostics

Roman Gerbers, Wilke Foellscher, Hong Chen, Constantine Anagnostopoulos, Mohammad Faghri

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Currently, the Lateral flow Immunoassays (LFIAs) are not able to perform complex multi-step immunodetection tests because of their inability to introduce multiple reagents in a controlled manner to the detection area autonomously. In this research, a point-of-care (POC) paper-based lateral flow immunosensor was developed incorporating a novel microfluidic valve technology. Layers of paper and tape were used to create a three-dimensional structure to form the fluidic network. Unlike the existing LFIAs, multiple directional valves are embedded in the test strip layers to control the order and the timing of mixing for the sample and multiple reagents. In this paper, we report a four-valve device which autonomously directs three different fluids to flow sequentially over the detection area. As proof of concept, a three-step alkaline phosphatase based Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol with Rabbit IgG as the model analyte was conducted to prove the suitability of the device for immunoassays. The detection limit of about 4.8 fm was obtained.

Original languageEnglish (US)
Pages (from-to)4042-4049
Number of pages8
JournalLab on a Chip
Volume14
Issue number20
DOIs
StatePublished - Oct 21 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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    Gerbers, R., Foellscher, W., Chen, H., Anagnostopoulos, C., & Faghri, M. (2014). A new paper-based platform technology for point-of-care diagnostics. Lab on a Chip, 14(20), 4042-4049. https://doi.org/10.1039/c4lc00786g