A novel creb family gene telomeric of HLA-DRA in the HLA complex

J. Min, H. Shukla, H. Kozono, S. K. Bronson, S. M. Weissman, D. D. Chaplin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

cDNA selection was used to identify genes encoded by a 440-kb yeast artificial chromosome (YAC) clone that spanned from HLA-DRA to CYP21 in the HLA complex. An initially selected short cDNA was used to isolate a 2639- nucleotide, apparently full-length cDNA from a human tonsil library. This cDNA contained one extended open reading frame that predicted a protein of 700 amino acids with a basic region and a leucine zipper that is highly similar to members of the Creb/ATF subfamily. High-stringency Southern blotting of total human genomic DNA using this cDNA as the probe showed only a single locus that mapped to the selecting YAC clone. This gene, designated Creb-related protein (Creb-rp), is expressed ubiquitously and is evolutionarily conserved in mammals. It is located in the HLA Class III region 6-10 kb centromeric of the XB gene, which encodes a tenascin-like extracellular matrix protein. Homologous sequences are located in the Class II-Class III interval of the mouse H-2 complex. The amino acid sequence homology and general structural features of the predicted protein indicate that this gene encodes a general transcription factor belonging to the Creb/ATF subfamily of the bZip superfamily.

Original languageEnglish (US)
Pages (from-to)149-156
Number of pages8
JournalGenomics
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

HLA-DR alpha-Chains
Complementary DNA
Yeast Artificial Chromosomes
Genes
Clone Cells
General Transcription Factors
Tenascin
Amino Acid Sequence Homology
Leucine Zippers
Proteins
Extracellular Matrix Proteins
Palatine Tonsil
Sequence Homology
Southern Blotting
Open Reading Frames
Mammals
Nucleotides
Amino Acids
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Min, J., Shukla, H., Kozono, H., Bronson, S. K., Weissman, S. M., & Chaplin, D. D. (1995). A novel creb family gene telomeric of HLA-DRA in the HLA complex. Genomics, 30(2), 149-156. https://doi.org/10.1006/geno.1995.9891
Min, J. ; Shukla, H. ; Kozono, H. ; Bronson, S. K. ; Weissman, S. M. ; Chaplin, D. D. / A novel creb family gene telomeric of HLA-DRA in the HLA complex. In: Genomics. 1995 ; Vol. 30, No. 2. pp. 149-156.
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Min, J, Shukla, H, Kozono, H, Bronson, SK, Weissman, SM & Chaplin, DD 1995, 'A novel creb family gene telomeric of HLA-DRA in the HLA complex', Genomics, vol. 30, no. 2, pp. 149-156. https://doi.org/10.1006/geno.1995.9891

A novel creb family gene telomeric of HLA-DRA in the HLA complex. / Min, J.; Shukla, H.; Kozono, H.; Bronson, S. K.; Weissman, S. M.; Chaplin, D. D.

In: Genomics, Vol. 30, No. 2, 01.01.1995, p. 149-156.

Research output: Contribution to journalArticle

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Min J, Shukla H, Kozono H, Bronson SK, Weissman SM, Chaplin DD. A novel creb family gene telomeric of HLA-DRA in the HLA complex. Genomics. 1995 Jan 1;30(2):149-156. https://doi.org/10.1006/geno.1995.9891