Abstract
We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) realtime UV dose (fluence) determination; and 2) simple actinometric determination of the Reactor Factor (RF = 1.06) that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This RF replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1395 cm 2/mJ. Aspergillus niger spores exhibited two-stage decay with a shoulder, with the resistant fraction of the population (f) of 0.251, k 1=0.1968 cm2/mJ, k2=0.0426 cm2/mJ.
| Original language | English (US) |
|---|---|
| Title of host publication | 10th International Conference on Healthy Buildings 2012 |
| Pages | 322-327 |
| Number of pages | 6 |
| Volume | 1 |
| State | Published - 2012 |
| Event | 10th International Conference on Healthy Buildings 2012 - Brisbane, QLD, Australia Duration: Jul 8 2012 → Jul 12 2012 |
Other
| Other | 10th International Conference on Healthy Buildings 2012 |
|---|---|
| Country | Australia |
| City | Brisbane, QLD |
| Period | 7/8/12 → 7/12/12 |
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All Science Journal Classification (ASJC) codes
- Civil and Structural Engineering
Cite this
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A novel dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions. / Martin, Stephen B.; Shogren, Elizabeth S.; Blum, David H.; Kremer, Paul A.; Bahnfleth, William P.; Freihaut, James.
10th International Conference on Healthy Buildings 2012. Vol. 1 2012. p. 322-327.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - A novel dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions
AU - Martin, Stephen B.
AU - Shogren, Elizabeth S.
AU - Blum, David H.
AU - Kremer, Paul A.
AU - Bahnfleth, William P.
AU - Freihaut, James
PY - 2012
Y1 - 2012
N2 - We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) realtime UV dose (fluence) determination; and 2) simple actinometric determination of the Reactor Factor (RF = 1.06) that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This RF replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1395 cm 2/mJ. Aspergillus niger spores exhibited two-stage decay with a shoulder, with the resistant fraction of the population (f) of 0.251, k 1=0.1968 cm2/mJ, k2=0.0426 cm2/mJ.
AB - We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) realtime UV dose (fluence) determination; and 2) simple actinometric determination of the Reactor Factor (RF = 1.06) that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This RF replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1395 cm 2/mJ. Aspergillus niger spores exhibited two-stage decay with a shoulder, with the resistant fraction of the population (f) of 0.251, k 1=0.1968 cm2/mJ, k2=0.0426 cm2/mJ.
UR - http://www.scopus.com/inward/record.url?scp=84883335289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883335289&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883335289
SN - 9781627480758
VL - 1
SP - 322
EP - 327
BT - 10th International Conference on Healthy Buildings 2012
ER -