A novel dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions

Stephen B. Martin, Elizabeth S. Shogren, David H. Blum, Paul A. Kremer, William P. Bahnfleth, James Freihaut

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) realtime UV dose (fluence) determination; and 2) simple actinometric determination of the Reactor Factor (RF = 1.06) that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This RF replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1395 cm 2/mJ. Aspergillus niger spores exhibited two-stage decay with a shoulder, with the resistant fraction of the population (f) of 0.251, k 1=0.1968 cm2/mJ, k2=0.0426 cm2/mJ.

Original languageEnglish (US)
Title of host publication10th International Conference on Healthy Buildings 2012
Pages322-327
Number of pages6
Volume1
StatePublished - 2012
Event10th International Conference on Healthy Buildings 2012 - Brisbane, QLD, Australia
Duration: Jul 8 2012Jul 12 2012

Other

Other10th International Conference on Healthy Buildings 2012
CountryAustralia
CityBrisbane, QLD
Period7/8/127/12/12

All Science Journal Classification (ASJC) codes

  • Civil and Structural Engineering

Fingerprint Dive into the research topics of 'A novel dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions'. Together they form a unique fingerprint.

  • Cite this

    Martin, S. B., Shogren, E. S., Blum, D. H., Kremer, P. A., Bahnfleth, W. P., & Freihaut, J. (2012). A novel dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions. In 10th International Conference on Healthy Buildings 2012 (Vol. 1, pp. 322-327)