A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

Michael P. Coleman, Helen J. Ambrose, Laura Carrel, Andrea H. Németh, Huntington F. Willard, Kay E. Davies

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalGenomics
Volume31
Issue number1
DOIs
StatePublished - Jan 1 1996

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X Chromosome Inactivation
Complementary DNA
Genes
Chromosomes, Human, X
X-Linked Genes
Hybrid Cells
Sequence Homology
Sequence Analysis
Mammals
Cell Line
Messenger RNA
Brain

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Coleman, Michael P. ; Ambrose, Helen J. ; Carrel, Laura ; Németh, Andrea H. ; Willard, Huntington F. ; Davies, Kay E. / A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status. In: Genomics. 1996 ; Vol. 31, No. 1. pp. 135-138.
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A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status. / Coleman, Michael P.; Ambrose, Helen J.; Carrel, Laura; Németh, Andrea H.; Willard, Huntington F.; Davies, Kay E.

In: Genomics, Vol. 31, No. 1, 01.01.1996, p. 135-138.

Research output: Contribution to journalArticle

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AU - Coleman, Michael P.

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