A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

Michael P. Coleman, Helen J. Ambrose, Laura Carrel, Andrea H. Németh, Huntington F. Willard, Kay E. Davies

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalGenomics
Volume31
Issue number1
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Genetics

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