A Novel Heterobifunctional Linker for Formyl to Thiol Coupling

Ned D. Heindel, Huiru Zhao, Roger Egolf, Chien Hsing Chang, Keith J. Schray, Jacqueline G. Emrich, Joanne P. McLaughlin, David V. Woo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17–1A, or an Fab′ derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.

Original languageEnglish (US)
Pages (from-to)427-430
Number of pages4
JournalBioconjugate Chemistry
Volume2
Issue number6
DOIs
StatePublished - May 1 1991

Fingerprint

ellipticine
Dextran
Dextrans
Sulfhydryl Compounds
Antineoplastic Agents
Tumors
Colorectal Neoplasms
Monoclonal Antibodies
Cells
Antigens
Monoclonal antibodies
Antibodies
Pharmaceutical Preparations
dextran dialdehyde
maleimide

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Heindel, N. D., Zhao, H., Egolf, R., Chang, C. H., Schray, K. J., Emrich, J. G., ... Woo, D. V. (1991). A Novel Heterobifunctional Linker for Formyl to Thiol Coupling. Bioconjugate Chemistry, 2(6), 427-430. https://doi.org/10.1021/bc00012a008
Heindel, Ned D. ; Zhao, Huiru ; Egolf, Roger ; Chang, Chien Hsing ; Schray, Keith J. ; Emrich, Jacqueline G. ; McLaughlin, Joanne P. ; Woo, David V. / A Novel Heterobifunctional Linker for Formyl to Thiol Coupling. In: Bioconjugate Chemistry. 1991 ; Vol. 2, No. 6. pp. 427-430.
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Heindel, ND, Zhao, H, Egolf, R, Chang, CH, Schray, KJ, Emrich, JG, McLaughlin, JP & Woo, DV 1991, 'A Novel Heterobifunctional Linker for Formyl to Thiol Coupling', Bioconjugate Chemistry, vol. 2, no. 6, pp. 427-430. https://doi.org/10.1021/bc00012a008

A Novel Heterobifunctional Linker for Formyl to Thiol Coupling. / Heindel, Ned D.; Zhao, Huiru; Egolf, Roger; Chang, Chien Hsing; Schray, Keith J.; Emrich, Jacqueline G.; McLaughlin, Joanne P.; Woo, David V.

In: Bioconjugate Chemistry, Vol. 2, No. 6, 01.05.1991, p. 427-430.

Research output: Contribution to journalArticle

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Heindel ND, Zhao H, Egolf R, Chang CH, Schray KJ, Emrich JG et al. A Novel Heterobifunctional Linker for Formyl to Thiol Coupling. Bioconjugate Chemistry. 1991 May 1;2(6):427-430. https://doi.org/10.1021/bc00012a008