A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression

Raghuvir S. Tomar, James N. Psathas, Hesheng Zhang, Zhengjian Zhang, Joseph C. Reese

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Gene expression depends upon the antagonistic actions of chromatin remodeling complexes. While this has been studied extensively for the enzymes that covalently modify the tails of histones, the mechanism of how ATP-dependent remodeling complexes antagonize each other to maintain the proper level of gene activity is not known. The gene encoding a large subunit of ribonucleotide reductase, RNR3, is regulated by ISW2 and SWI/SNF, complexes that repress and activate transcription, respectively. Here, we studied the functional interactions of these two complexes at RNR3. Deletion of ISW2 causes constitutive recruitment of SWI/SNF, and conditional reexpression of ISW2 causes the repositioning of nucleosomes and reduced SWI/SNF occupancy at RNR3. Thus, ISW2 is required for restriction of access of SWI/SNF to the RNR3 promoter under the uninduced condition. Interestingly, the binding of sequence-specific DNA binding factors and the general transcription machinery are unaffected by the status of ISW2, suggesting that disruption of nucleosome positioning does not cause a nonspecific increase in cross-linking of all factors to RNR3. We provide evidence that ISW2 does not act on SWI/SNF directly but excludes its occupancy by positioning nucleosomes over the promoter. Genetic disruption of nucleosome positioning by other means led to a similar phenotype, linking repressed chromatin structure to SWI/SNF exclusion. Thus, incorporation of promoters into a repressive chromatin structure is essential for prevention of the opportunistic actions of nucleosome-disrupting activities in vivo, providing a novel mechanism for maintaining tight control of gene expression.

Original languageEnglish (US)
Pages (from-to)3255-3265
Number of pages11
JournalMolecular and cellular biology
Volume29
Issue number12
DOIs
StatePublished - Jun 1 2009

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Chromatin Assembly and Disassembly
Nucleosomes
Adenosine Triphosphate
Chromatin
General Transcription Factors
Ribonucleotide Reductases
Gene Expression
Histones
Genes
Phenotype
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Tomar, Raghuvir S. ; Psathas, James N. ; Zhang, Hesheng ; Zhang, Zhengjian ; Reese, Joseph C. / A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression. In: Molecular and cellular biology. 2009 ; Vol. 29, No. 12. pp. 3255-3265.
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A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression. / Tomar, Raghuvir S.; Psathas, James N.; Zhang, Hesheng; Zhang, Zhengjian; Reese, Joseph C.

In: Molecular and cellular biology, Vol. 29, No. 12, 01.06.2009, p. 3255-3265.

Research output: Contribution to journalArticle

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AU - Psathas, James N.

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