A novel method for site specific introduction of single model oxidative DNA lesions into oligodeoxyribonucleotides

Zafer Hatahet, Andrei A. Purmal, Susan S. Wallace

Research output: Contribution to journalArticle

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Calf thymus terminal deoxynucleotidyl transferase was used to incorporate several products of oxidative base damage onto the 3′ end of oilgodeoxyribonucleotides. Under the defined conditions described in this report, single residues of dihydrothymine, β-ureidoisobutyric acid, thymine glycol, urea, 7-hydro-8-oxoadenine, 7-hydro-8-oxoguanine, 5-hydroxycytosine and 5-hydroxyuracil were incorporated into oligodeoxyribonucleotides of different lengths. The reaction is both efficient and cost effective. The 3′ termini of the reaction products were suitable substrates for ligation by phage T4 DNA ligase, facilitating greatly the construction of oligodeoxyribonucleotides containing unique and site specific oxidative DNA lesions.

Original languageEnglish (US)
Pages (from-to)1563-1568
Number of pages6
JournalNucleic acids research
Issue number7
Publication statusPublished - Apr 11 1993


All Science Journal Classification (ASJC) codes

  • Genetics

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