A novel method for viral gene delivery in solid tumors

Yong Wang, Shanling Liu, Chuan Yuan Li, Fan Yuan

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Intratumoral infusion is the most commonly used method for viral gene delivery in clinical trials for cancer treatment. However, a potential problem in this approach is that viral vectors may disseminate from tumor to normal tissues during and after the infusion. To reduce the dissemination, we developed a novel method based on a biocompatible polymer, poloxamer 407, which could significantly increase the viscosity of virus suspension when the temperature was changed from 4°C to 37°C. With this method, we could significantly increase transgene expression in solid tumors and reduce virus dissemination by 2 orders of magnitude after intratumoral infusion of adenoviral vectors. The mechanism of reduction was likely to be that the viscous poloxamer solution blocked convection of viral vectors in the interstitial space and the lumen of microvessels in the vicinity of the infusion site. This method has a potential to be used in the clinic for enhancing efficacy and reducing toxicity in viral gene therapy.

Original languageEnglish (US)
Pages (from-to)7541-7545
Number of pages5
JournalCancer Research
Volume65
Issue number17
DOIs
StatePublished - Sep 1 2005

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Viral Genes
Poloxamer
Neoplasms
Oncogenic Viruses
Convection
Microvessels
Transgenes
Viscosity
Genetic Therapy
Suspensions
Polymers
Clinical Trials
Viruses
Temperature
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Wang, Yong ; Liu, Shanling ; Li, Chuan Yuan ; Yuan, Fan. / A novel method for viral gene delivery in solid tumors. In: Cancer Research. 2005 ; Vol. 65, No. 17. pp. 7541-7545.
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A novel method for viral gene delivery in solid tumors. / Wang, Yong; Liu, Shanling; Li, Chuan Yuan; Yuan, Fan.

In: Cancer Research, Vol. 65, No. 17, 01.09.2005, p. 7541-7545.

Research output: Contribution to journalArticle

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