A novel pre-clinical murine model to study the life cycle and progression of cervical and anal papillomavirus infections

Nancy M. Cladel, Lynn R. Budgeon, Karla K. Balogh, Timothy Cooper, Jiafen Hu, Neil Christensen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Papillomavirus disease and associated cancers remain a significant health burden in much of the world. The current protective vaccines, Gardasil and Cervarix, are expensive and not readily available to the underprivileged. In addition, the vaccines have not gained wide acceptance in the United States nor do they provide therapeutic value. Papillomaviruses are strictly species specific and thus human viruses cannot be studied in an animal host. An appropriate model for mucosal disease has long been sought. We chose to investigate whether the newly discovered mouse papillomavirus, MmuPV1, could infect mucosal tissues in Foxn1nu/Foxn1nu mice. Methods: The vaginal and anal canals of Foxn1nu/Foxn1nu mice were gently abraded using Nonoxy-nol-9 and "Doctor's BrushPicks " and MmuPV1 was delivered into the vaginal tract or the anal canal. Results: Productive vaginal, cervical and anal infections developed in all mice. Vaginal/cervical infections could be monitored by vaginal lavage. Dysplasias were evident in all animals. Conclusions: Anogenital tissues of a common laboratory mouse can be infected with a papillomavirus unique to that animal. This observation will pave the way for fundamental virological and immunological studies that have been challenging to carry out heretofore due to lack of a suitable model system.

Original languageEnglish (US)
Article numbere0120128
JournalPloS one
Volume10
Issue number3
DOIs
StatePublished - Mar 24 2015

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Papillomavirus Infections
Papillomaviridae
Life Cycle Stages
Life cycle
life cycle (organisms)
Animals
animal models
Canals
mice
Vaccines
infection
Anal Canal
Viruses
Vaginal Douching
vaccines
vertebrate viruses
animals
Health
Tissue
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "A novel pre-clinical murine model to study the life cycle and progression of cervical and anal papillomavirus infections",
abstract = "Background: Papillomavirus disease and associated cancers remain a significant health burden in much of the world. The current protective vaccines, Gardasil and Cervarix, are expensive and not readily available to the underprivileged. In addition, the vaccines have not gained wide acceptance in the United States nor do they provide therapeutic value. Papillomaviruses are strictly species specific and thus human viruses cannot be studied in an animal host. An appropriate model for mucosal disease has long been sought. We chose to investigate whether the newly discovered mouse papillomavirus, MmuPV1, could infect mucosal tissues in Foxn1nu/Foxn1nu mice. Methods: The vaginal and anal canals of Foxn1nu/Foxn1nu mice were gently abraded using Nonoxy-nol-9 and {"}Doctor's BrushPicks {"} and MmuPV1 was delivered into the vaginal tract or the anal canal. Results: Productive vaginal, cervical and anal infections developed in all mice. Vaginal/cervical infections could be monitored by vaginal lavage. Dysplasias were evident in all animals. Conclusions: Anogenital tissues of a common laboratory mouse can be infected with a papillomavirus unique to that animal. This observation will pave the way for fundamental virological and immunological studies that have been challenging to carry out heretofore due to lack of a suitable model system.",
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A novel pre-clinical murine model to study the life cycle and progression of cervical and anal papillomavirus infections. / Cladel, Nancy M.; Budgeon, Lynn R.; Balogh, Karla K.; Cooper, Timothy; Hu, Jiafen; Christensen, Neil.

In: PloS one, Vol. 10, No. 3, e0120128, 24.03.2015.

Research output: Contribution to journalArticle

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AU - Hu, Jiafen

AU - Christensen, Neil

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