A novel preparation of degradable scaffolds using BSA microbubbles as porogen

Ashwin Nair, Jian Yang, Liping Tang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.

Original languageEnglish (US)
Title of host publication2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
Pages31-34
Number of pages4
DOIs
StatePublished - Dec 1 2007
Event2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS - Richardson, TX, United States
Duration: Nov 11 2007Nov 12 2007

Other

Other2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
CountryUnited States
CityRichardson, TX
Period11/11/0711/12/07

Fingerprint

Microbubbles
Scaffolds
Proteins
Cryoultramicrotomy
Drug Carriers
Porosity
Bovine Serum Albumin
Hydrophobic and Hydrophilic Interactions
Chemokines
Cell Movement
Regeneration
Albumins
Nitrogen
Gases
Medicine
Coatings
Scaffolds (biology)
Hydrophobicity
Phase separation
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nair, A., Yang, J., & Tang, L. (2007). A novel preparation of degradable scaffolds using BSA microbubbles as porogen. In 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS (pp. 31-34). [4454166] https://doi.org/10.1109/EMBSW.2007.4454166
Nair, Ashwin ; Yang, Jian ; Tang, Liping. / A novel preparation of degradable scaffolds using BSA microbubbles as porogen. 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS. 2007. pp. 31-34
@inproceedings{465730bf4a3540958298bdadbc4ea5a3,
title = "A novel preparation of degradable scaffolds using BSA microbubbles as porogen",
abstract = "Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.",
author = "Ashwin Nair and Jian Yang and Liping Tang",
year = "2007",
month = "12",
day = "1",
doi = "10.1109/EMBSW.2007.4454166",
language = "English (US)",
isbn = "9781424416264",
pages = "31--34",
booktitle = "2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS",

}

Nair, A, Yang, J & Tang, L 2007, A novel preparation of degradable scaffolds using BSA microbubbles as porogen. in 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS., 4454166, pp. 31-34, 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS, Richardson, TX, United States, 11/11/07. https://doi.org/10.1109/EMBSW.2007.4454166

A novel preparation of degradable scaffolds using BSA microbubbles as porogen. / Nair, Ashwin; Yang, Jian; Tang, Liping.

2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS. 2007. p. 31-34 4454166.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - A novel preparation of degradable scaffolds using BSA microbubbles as porogen

AU - Nair, Ashwin

AU - Yang, Jian

AU - Tang, Liping

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.

AB - Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.

UR - http://www.scopus.com/inward/record.url?scp=48949118111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48949118111&partnerID=8YFLogxK

U2 - 10.1109/EMBSW.2007.4454166

DO - 10.1109/EMBSW.2007.4454166

M3 - Conference contribution

AN - SCOPUS:48949118111

SN - 9781424416264

SP - 31

EP - 34

BT - 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS

ER -

Nair A, Yang J, Tang L. A novel preparation of degradable scaffolds using BSA microbubbles as porogen. In 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS. 2007. p. 31-34. 4454166 https://doi.org/10.1109/EMBSW.2007.4454166