Abstract
Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.
| Original language | English (US) |
|---|---|
| Title of host publication | 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS |
| Pages | 31-34 |
| Number of pages | 4 |
| DOIs | |
| State | Published - Dec 1 2007 |
| Event | 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS - Richardson, TX, United States Duration: Nov 11 2007 → Nov 12 2007 |
Other
| Other | 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS |
|---|---|
| Country | United States |
| City | Richardson, TX |
| Period | 11/11/07 → 11/12/07 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
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A novel preparation of degradable scaffolds using BSA microbubbles as porogen. / Nair, Ashwin; Yang, Jian; Tang, Liping.
2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS. 2007. p. 31-34 4454166.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - A novel preparation of degradable scaffolds using BSA microbubbles as porogen
AU - Nair, Ashwin
AU - Yang, Jian
AU - Tang, Liping
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.
AB - Biodegradable scaffolds play a key role in contemporary medicine for tissue replacement as well as regeneration. Many degradable scaffold fabrication techniques have been developed. However, these scaffolds often hamper the growth of cells due to material hydrophobicity and/or lack of biocompatible protein coating. To overcome the common problems, in this study, the use of protein microbubbles as a porogen and drug/protein carrier to produce polymeric scaffolds with good porosity was conceptualized. Albumin bubbles were produced by sonicating bovine serum albumin in the presence of nitrogen gas. PLGA scaffolds were then prepared by thermally induced phase separation with the incorporation of protein microbubbles as porogens. SEM and cryosectioning of scaffold revealed the presence of open interconnected pores measuring around 100 to 150 μm size, which is suitable for cell migration into scaffold. This novel technique provides two distinct advantages. First, microbubbles are made of biological materials which can provide biocompatible protein coating on the pores throughout the scaffold. Second, apart from having produced scaffolds with larger pores compared to conventional methods, our novel scaffold also has the potential to function as a delivery mechanism for delivering chemokines and drugs into the polymeric matrix.
UR - http://www.scopus.com/inward/record.url?scp=48949118111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48949118111&partnerID=8YFLogxK
U2 - 10.1109/EMBSW.2007.4454166
DO - 10.1109/EMBSW.2007.4454166
M3 - Conference contribution
AN - SCOPUS:48949118111
SN - 9781424416264
SP - 31
EP - 34
BT - 2007 IEEE Dallas Engineering in Medicine and Biology Workshop, DEMBS
ER -