A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle

Wansheng Liu, Yaqi Zhao, Chen Lu, Gang Ning, Yun Ma, Francisco Javier Diaz, Michael O'Connor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen that is predominantly expressed in normal testicular tissues and a variety of tumors. The function of the PRAME family in spermatogenesis remains unknown. This study was designed to characterize the Y-linked PRAME (PRAMEY) protein during spermatogenesis in cattle. We found that PRAMEY is a novel male germ cell-specific, and a germinal granule-associated protein that is expressed in spermatogenic cells during spermatogenesis. The intact PRAMEY protein (58 kDa) was detected in different ages of testes but not in epididymal spermatozoa. A PRAMEY isoform (30 kDa) was highly expressed only in testes after puberty and in epididymal spermatozoa. This isoform interacts with PP1?2 and is likely the mature protein present in the testes and sperm. Immunofuorescent staining demonstrated that PRAMEY was located predominantly in the acrosome granule of spermatids, and in acrosome and fagellum of spermatozoa. Immunogold electron microscopy further localized the PRAMEY protein complex to the nucleus and several cytoplasmic organelles, including the rough endoplasmic reticulum, some small vesicles, the intermitochondrial cement, the chromatoid body and the centrioles, in spermatogonia, spermatocytes, spermatids and/or spermatozoa. PRAMEY was highly enriched in and structurally associated with the matrix of the acrosomal granule (AG) in round spermatids, and migrated with the expansion of the AG during acrosomal biogenesis. While the function of PRAMEY remains unclear during spermatogenesis, our results suggest that PRAMEY may play an essential role in acrosome biogenesis and spermatogenesis.

Original languageEnglish (US)
Pages (from-to)847-863
Number of pages17
JournalReproduction
Volume153
Issue number6
DOIs
StatePublished - Jan 1 2017

Fingerprint

Spermatogenesis
Spermatozoa
Testis
Melanoma-Specific Antigens
Acrosome
Spermatids
Proteins
Protein Isoforms
Centrioles
Spermatogonia
Spermatocytes
Rough Endoplasmic Reticulum
Testicular Neoplasms
Puberty
Germ Cells
Organelles
Electron Microscopy
Staining and Labeling
Antigens
Neoplasms

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

Cite this

Liu, Wansheng ; Zhao, Yaqi ; Lu, Chen ; Ning, Gang ; Ma, Yun ; Diaz, Francisco Javier ; O'Connor, Michael. / A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle. In: Reproduction. 2017 ; Vol. 153, No. 6. pp. 847-863.
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abstract = "Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen that is predominantly expressed in normal testicular tissues and a variety of tumors. The function of the PRAME family in spermatogenesis remains unknown. This study was designed to characterize the Y-linked PRAME (PRAMEY) protein during spermatogenesis in cattle. We found that PRAMEY is a novel male germ cell-specific, and a germinal granule-associated protein that is expressed in spermatogenic cells during spermatogenesis. The intact PRAMEY protein (58 kDa) was detected in different ages of testes but not in epididymal spermatozoa. A PRAMEY isoform (30 kDa) was highly expressed only in testes after puberty and in epididymal spermatozoa. This isoform interacts with PP1?2 and is likely the mature protein present in the testes and sperm. Immunofuorescent staining demonstrated that PRAMEY was located predominantly in the acrosome granule of spermatids, and in acrosome and fagellum of spermatozoa. Immunogold electron microscopy further localized the PRAMEY protein complex to the nucleus and several cytoplasmic organelles, including the rough endoplasmic reticulum, some small vesicles, the intermitochondrial cement, the chromatoid body and the centrioles, in spermatogonia, spermatocytes, spermatids and/or spermatozoa. PRAMEY was highly enriched in and structurally associated with the matrix of the acrosomal granule (AG) in round spermatids, and migrated with the expansion of the AG during acrosomal biogenesis. While the function of PRAMEY remains unclear during spermatogenesis, our results suggest that PRAMEY may play an essential role in acrosome biogenesis and spermatogenesis.",
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A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle. / Liu, Wansheng; Zhao, Yaqi; Lu, Chen; Ning, Gang; Ma, Yun; Diaz, Francisco Javier; O'Connor, Michael.

In: Reproduction, Vol. 153, No. 6, 01.01.2017, p. 847-863.

Research output: Contribution to journalArticle

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AU - Zhao, Yaqi

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AU - Ning, Gang

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AU - Diaz, Francisco Javier

AU - O'Connor, Michael

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