A novel transforming growth factor-β receptor-interacting protein that is also a light chain of the motor protein dynein

Qian Tang, Cory M. Staub, Guofeng Gao, Qunyan Jin, Zhengke Wang, Wei Ding, Rosemarie E. Aurigemma, Kathleen Mulder

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The phosphorylated, activated cytoplasmic domains of the transforming growth factor-β (TGFβ) receptors were used as probes to screen an expression library that was prepared from a highly TGFβ-responsive intestinal epithelial cell line. One of the TGFβ receptor-interacting proteins isolated was identified to be the mammalian homologue of the LC7 family (mLC7) of dynein light chains (DLCs). This 11-kDa cytoplasmic protein interacts with the TGFβ receptor complex intracellularly and is phosphorylated on serine residues after ligand-receptor engagement. Forced expression of mLC7-1 induces specific TGFβ responses, including an activation of Jun N-terminal kinase (JNK), a phosphorylation of c-Jun, and an inhibition of cell growth. Furthermore, TGFβ induces the recruitment of mLC7-1 to the intermediate chain of dynein. A kinase-deficient form of TGFβ RII prevents both mLC7-1 phosphorylation and interaction with the dynein intermediate chain (DIC). This is the first demonstration of a link between cytoplasmic dynein and a natural growth inhibitory cytokine. Furthermore, our results suggest that TGFβ pathway components may use a motor protein light chain as a receptor for the recruitment and transport of specific cargo along microtublules.

Original languageEnglish (US)
Pages (from-to)4484-4496
Number of pages13
JournalMolecular biology of the cell
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2002

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Receptor-Interacting Protein Serine-Threonine Kinases
Dyneins
Growth Factor Receptors
Transforming Growth Factors
Light
Proteins
Phosphotransferases
Cytoplasmic Dyneins
Phosphorylation
Growth
Serine
Epithelial Cells
Cytokines
Ligands
Cell Line

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Tang, Qian ; Staub, Cory M. ; Gao, Guofeng ; Jin, Qunyan ; Wang, Zhengke ; Ding, Wei ; Aurigemma, Rosemarie E. ; Mulder, Kathleen. / A novel transforming growth factor-β receptor-interacting protein that is also a light chain of the motor protein dynein. In: Molecular biology of the cell. 2002 ; Vol. 13, No. 12. pp. 4484-4496.
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A novel transforming growth factor-β receptor-interacting protein that is also a light chain of the motor protein dynein. / Tang, Qian; Staub, Cory M.; Gao, Guofeng; Jin, Qunyan; Wang, Zhengke; Ding, Wei; Aurigemma, Rosemarie E.; Mulder, Kathleen.

In: Molecular biology of the cell, Vol. 13, No. 12, 01.12.2002, p. 4484-4496.

Research output: Contribution to journalArticle

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AU - Tang, Qian

AU - Staub, Cory M.

AU - Gao, Guofeng

AU - Jin, Qunyan

AU - Wang, Zhengke

AU - Ding, Wei

AU - Aurigemma, Rosemarie E.

AU - Mulder, Kathleen

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