A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics

Reza Riahi, Joseph C. Liao, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In this paper, a novel approach for rapid, species-specific detection of pathogens via bacterial 16S rRNA is demonstrated using a microparticle enhanced double-stranded DNA (dsDNA) probe for point-of-care diagnostics. By incorporating strep-tavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay is improved by three orders of magnitude and can detect as few as eight bacteria without target amplification. The assay requires less than 40 min compared to days in standard procedure. The probe is highly specific against common uropathogens and is inert to the matrix effects in urine samples from patients with urinary tract infections (UTI).

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1944-1946
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Assays
Pathogens
Amplification
Bacteria

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Riahi, R., Liao, J. C., & Wong, P. K. (2011). A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1944-1946). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).
Riahi, Reza ; Liao, Joseph C. ; Wong, Pak Kin. / A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1944-1946 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "In this paper, a novel approach for rapid, species-specific detection of pathogens via bacterial 16S rRNA is demonstrated using a microparticle enhanced double-stranded DNA (dsDNA) probe for point-of-care diagnostics. By incorporating strep-tavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay is improved by three orders of magnitude and can detect as few as eight bacteria without target amplification. The assay requires less than 40 min compared to days in standard procedure. The probe is highly specific against common uropathogens and is inert to the matrix effects in urine samples from patients with urinary tract infections (UTI).",
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Riahi, R, Liao, JC & Wong, PK 2011, A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 1944-1946, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics. / Riahi, Reza; Liao, Joseph C.; Wong, Pak Kin.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1944-1946 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Riahi R, Liao JC, Wong PK. A particle-enhanced double-stranded DNA probe for rapid detection of bacterial 16S rRNA toward urinary tract infection diagnostics. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1944-1946. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).