A perspective on enzyme catalysis

Stephen J. Benkovic, Sharon Hammes-Schiffer

Research output: Contribution to journalReview article

801 Citations (Scopus)

Abstract

The seminal hypotheses proposed over the years for enzymatic catalysis are scrutinized. The historical record is explored from both biochemical and theoretical perspectives. Particular attention is given to the impact of molecular motions within the protein on the enzyme's catalytic properties. A case study for the enzyme dihydrofolate reductase provides evidence for coupled networks of predominantly conserved residues that influence the protein structure and motion. Such coupled networks have important implications for the origin and evolution of enzymes, as well as for protein engineering.

Original languageEnglish (US)
Pages (from-to)1196-1202
Number of pages7
JournalScience
Volume301
Issue number5637
DOIs
StatePublished - Aug 29 2003

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Catalysis
Enzymes
Protein Engineering
Tetrahydrofolate Dehydrogenase
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Benkovic, S. J., & Hammes-Schiffer, S. (2003). A perspective on enzyme catalysis. Science, 301(5637), 1196-1202. https://doi.org/10.1126/science.1085515
Benkovic, Stephen J. ; Hammes-Schiffer, Sharon. / A perspective on enzyme catalysis. In: Science. 2003 ; Vol. 301, No. 5637. pp. 1196-1202.
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Benkovic, SJ & Hammes-Schiffer, S 2003, 'A perspective on enzyme catalysis', Science, vol. 301, no. 5637, pp. 1196-1202. https://doi.org/10.1126/science.1085515

A perspective on enzyme catalysis. / Benkovic, Stephen J.; Hammes-Schiffer, Sharon.

In: Science, Vol. 301, No. 5637, 29.08.2003, p. 1196-1202.

Research output: Contribution to journalReview article

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Benkovic SJ, Hammes-Schiffer S. A perspective on enzyme catalysis. Science. 2003 Aug 29;301(5637):1196-1202. https://doi.org/10.1126/science.1085515