A physical map of the Chinese chestnut (Castanea mollissima) genome and its integration with the genetic map

Guang Chen Fang, Barbara P. Blackmon, Margaret E. Staton, C. Dana Nelson, Thomas L. Kubisiak, Bode A. Olukolu, David Henry, Tatyana Zhebentyayeva, Christopher A. Saski, Chun Huai Cheng, Megan Monsanto, Stephen Ficklin, Michael Atkins, Laura L. Georgi, Abdelali Barakat, Nicholas Wheeler, John E. Carlson, Ronald Sederoff, Albert G. Abbott

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www. fagaceae. org/physical_maps.

Original languageEnglish (US)
Pages (from-to)525-537
Number of pages13
JournalTree Genetics and Genomes
Volume9
Issue number2
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Forestry
  • Molecular Biology
  • Genetics
  • Horticulture

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