A pilot study on the utility of reduced urine collection frequency protocols for the assessment of reproductive hormones in adolescent girls

Heather C.M. Allaway, Esther M. John, Theresa H. Keegan, Mary Jane De Souza

Research output: Contribution to journalArticle

Abstract

The objectives of this study were to assess the feasibility of and compliance to collecting urine samples in pre- and postmenarcheal girls and to determine if a less than daily collection frequency was sufficient for assessing ovarian function. Twenty-five postmenarcheal girls (11-17 years) collected samples using either a two or a three samples/week protocol during one menstrual cycle. Exposure and mean estrone-1-glucuronide (E1G) and pregnanediol glucuronide concentrations were calculated, and evidence of luteal activity (ELA) was evaluated. Sixteen premenarcheal girls (8-11 years) collected one sample/month for six consecutive months. Samples were analyzed for E1G concentration. Participant compliance was calculated using dates on the urine samples and paper calendars. Participants collecting three samples/week were more compliant to the protocol than those collecting two samples/week (83.6%±2.6% vs. 66.8%±6.6%; p=0.034). There were no differences (p>0.10) regarding paper calendar return (81.8%±12.2% vs. 92.9%±7.1%), recording menses (55.6%±17.6% vs. 92.3%±7.7%) or sample collection (88.9%±11.1% vs. 84.6%±10.4%) between the two protocols. The average cycle length was 30.5±1.3 days and 32% of cycles had ELA. The premenarcheal girls were 100% compliant to the protocol. Only 68.8% of participants returned the paper calendar and 81.8% of those participants recorded sample collection. The average E1G concentration was 15.9±3.8 ng/mL. Use of a less than daily collection frequency during one menstrual cycle in postmenarcheal, adolescent girls is feasible and provides informative data about ovarian function. Collection of one sample/month in premenarcheal girls is feasible and detects the expected low E1G concentrations. Alternate strategies to the use of a paper calendar should be considered.

Original languageEnglish (US)
Pages (from-to)1083-1093
Number of pages11
JournalJournal of Pediatric Endocrinology and Metabolism
Volume30
Issue number10
DOIs
StatePublished - Oct 26 2017

Fingerprint

Urine Specimen Collection
Glucuronides
Estrone
Hormones
Corpus Luteum
Menstrual Cycle
Pregnanediol
Urine
Menstruation
Compliance
Calendars

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

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title = "A pilot study on the utility of reduced urine collection frequency protocols for the assessment of reproductive hormones in adolescent girls",
abstract = "The objectives of this study were to assess the feasibility of and compliance to collecting urine samples in pre- and postmenarcheal girls and to determine if a less than daily collection frequency was sufficient for assessing ovarian function. Twenty-five postmenarcheal girls (11-17 years) collected samples using either a two or a three samples/week protocol during one menstrual cycle. Exposure and mean estrone-1-glucuronide (E1G) and pregnanediol glucuronide concentrations were calculated, and evidence of luteal activity (ELA) was evaluated. Sixteen premenarcheal girls (8-11 years) collected one sample/month for six consecutive months. Samples were analyzed for E1G concentration. Participant compliance was calculated using dates on the urine samples and paper calendars. Participants collecting three samples/week were more compliant to the protocol than those collecting two samples/week (83.6{\%}±2.6{\%} vs. 66.8{\%}±6.6{\%}; p=0.034). There were no differences (p>0.10) regarding paper calendar return (81.8{\%}±12.2{\%} vs. 92.9{\%}±7.1{\%}), recording menses (55.6{\%}±17.6{\%} vs. 92.3{\%}±7.7{\%}) or sample collection (88.9{\%}±11.1{\%} vs. 84.6{\%}±10.4{\%}) between the two protocols. The average cycle length was 30.5±1.3 days and 32{\%} of cycles had ELA. The premenarcheal girls were 100{\%} compliant to the protocol. Only 68.8{\%} of participants returned the paper calendar and 81.8{\%} of those participants recorded sample collection. The average E1G concentration was 15.9±3.8 ng/mL. Use of a less than daily collection frequency during one menstrual cycle in postmenarcheal, adolescent girls is feasible and provides informative data about ovarian function. Collection of one sample/month in premenarcheal girls is feasible and detects the expected low E1G concentrations. Alternate strategies to the use of a paper calendar should be considered.",
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A pilot study on the utility of reduced urine collection frequency protocols for the assessment of reproductive hormones in adolescent girls. / Allaway, Heather C.M.; John, Esther M.; Keegan, Theresa H.; De Souza, Mary Jane.

In: Journal of Pediatric Endocrinology and Metabolism, Vol. 30, No. 10, 26.10.2017, p. 1083-1093.

Research output: Contribution to journalArticle

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AB - The objectives of this study were to assess the feasibility of and compliance to collecting urine samples in pre- and postmenarcheal girls and to determine if a less than daily collection frequency was sufficient for assessing ovarian function. Twenty-five postmenarcheal girls (11-17 years) collected samples using either a two or a three samples/week protocol during one menstrual cycle. Exposure and mean estrone-1-glucuronide (E1G) and pregnanediol glucuronide concentrations were calculated, and evidence of luteal activity (ELA) was evaluated. Sixteen premenarcheal girls (8-11 years) collected one sample/month for six consecutive months. Samples were analyzed for E1G concentration. Participant compliance was calculated using dates on the urine samples and paper calendars. Participants collecting three samples/week were more compliant to the protocol than those collecting two samples/week (83.6%±2.6% vs. 66.8%±6.6%; p=0.034). There were no differences (p>0.10) regarding paper calendar return (81.8%±12.2% vs. 92.9%±7.1%), recording menses (55.6%±17.6% vs. 92.3%±7.7%) or sample collection (88.9%±11.1% vs. 84.6%±10.4%) between the two protocols. The average cycle length was 30.5±1.3 days and 32% of cycles had ELA. The premenarcheal girls were 100% compliant to the protocol. Only 68.8% of participants returned the paper calendar and 81.8% of those participants recorded sample collection. The average E1G concentration was 15.9±3.8 ng/mL. Use of a less than daily collection frequency during one menstrual cycle in postmenarcheal, adolescent girls is feasible and provides informative data about ovarian function. Collection of one sample/month in premenarcheal girls is feasible and detects the expected low E1G concentrations. Alternate strategies to the use of a paper calendar should be considered.

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