A plasma lithography microengineered assay for studying architecture dependent wound healing of endothelial cells

Yongliang Yang, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A plasma lithography microengineered wound healing assay is developed to study architecture and injury dependences of endothelial cell migration. We study the migration of endothelial cell monolayers with dimensions from single cell width to millimeters mimicking various cardiovascular structures. Furthermore, we investigate the role of mechanical injury using wound healing assays that introduce different degrees of cell injury. Remarkably, the endothelial migration rate is robustly maintained in various circumstances: line patterns with different widths and different levels of mechanical injury. Our results also reveal that the number of leader cells, which guide the healing process, is proportional to the width of the structure suggesting a novel multicellular mechanism for regulating the endothelial wound healing process.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages326-328
Number of pages3
Volume1
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Yang, Y., & Wong, P. K. (2011). A plasma lithography microengineered assay for studying architecture dependent wound healing of endothelial cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 1, pp. 326-328)