TY - GEN
T1 - A portable impedance biosensor instrument for rapid detection of avian influenza virus
AU - Lin, Jianhan
AU - Lum, Jacob
AU - Wang, Ronghui
AU - Tung, Steve
AU - Hargis, Billy
AU - Li, Yanbin
AU - Lu, Huaguang
AU - Berghman, Luc
PY - 2010/12/1
Y1 - 2010/12/1
N2 - The objectives of this study were to improve our previous portable impedance biosensor instrument with more rapid, reliable and quantitative features for detection of avian influenza (AI) virus and to evaluate its performance using H5N1 and H5N2 virus. A high-intensity and high-gradient magnetic field based separator was designed and fabricated for rapid separation of AI virus. KPL measuring solution was used as background medium to obtain higher stability. Twin BNC connection between the electronic circuit and the biochip was employed to prevent radio frequency interference. The amplitude limiting average filtering method was used to eliminate false signals and a linear regression model was built for the embedded control software. A data acquisition software was developed for communication and data processing. This impedance biosensor instrument could work stand-alone or be connected with a laptop via USB interface. The experimental results showed that the impedance biosensor could identify H5N1 virus with a detection limit of 103 EID50/mL in 30 min. A prototype of the improved impedance biosensor was fabricated for evaluation with cloacal swab samples from AI H5N2 virus infected chickens. Compared with viral isolation, this biosensor instrument had a false negative rate of 10%, whereas realtime RT-PCR showed a false negative/positive rate of 20%.
AB - The objectives of this study were to improve our previous portable impedance biosensor instrument with more rapid, reliable and quantitative features for detection of avian influenza (AI) virus and to evaluate its performance using H5N1 and H5N2 virus. A high-intensity and high-gradient magnetic field based separator was designed and fabricated for rapid separation of AI virus. KPL measuring solution was used as background medium to obtain higher stability. Twin BNC connection between the electronic circuit and the biochip was employed to prevent radio frequency interference. The amplitude limiting average filtering method was used to eliminate false signals and a linear regression model was built for the embedded control software. A data acquisition software was developed for communication and data processing. This impedance biosensor instrument could work stand-alone or be connected with a laptop via USB interface. The experimental results showed that the impedance biosensor could identify H5N1 virus with a detection limit of 103 EID50/mL in 30 min. A prototype of the improved impedance biosensor was fabricated for evaluation with cloacal swab samples from AI H5N2 virus infected chickens. Compared with viral isolation, this biosensor instrument had a false negative rate of 10%, whereas realtime RT-PCR showed a false negative/positive rate of 20%.
UR - http://www.scopus.com/inward/record.url?scp=79951934023&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951934023&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5690302
DO - 10.1109/ICSENS.2010.5690302
M3 - Conference contribution
AN - SCOPUS:79951934023
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 1558
EP - 1563
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -