A portable microfluidic paper-based device for elisa

X. Y. Liu, C. M. Cheng, A. W. Martinez, K. A. Mirica, X. J. Li, S. T. Phillips, M. Mascareñas, G. M. Whitesides

Research output: Chapter in Book/Report/Conference proceedingConference contribution

41 Citations (Scopus)

Abstract

This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

Original languageEnglish (US)
Title of host publication2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages75-78
Number of pages4
DOIs
StatePublished - Apr 13 2011
Event24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
Duration: Jan 23 2011Jan 27 2011

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
CountryMexico
CityCancun
Period1/23/111/27/11

Fingerprint

Microfluidics
Immunosorbents
Assays
Enzymes
reagents
enzymes
Buffers
Fluidics
Antigens
Hepatitis B Surface Antigens
buffers
hepatitis
Contamination
Immunoglobulin G
fluidics
rabbits
antigens
serums
strip
delivery

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Liu, X. Y., Cheng, C. M., Martinez, A. W., Mirica, K. A., Li, X. J., Phillips, S. T., ... Whitesides, G. M. (2011). A portable microfluidic paper-based device for elisa. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 75-78). [5734365] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734365
Liu, X. Y. ; Cheng, C. M. ; Martinez, A. W. ; Mirica, K. A. ; Li, X. J. ; Phillips, S. T. ; Mascareñas, M. ; Whitesides, G. M. / A portable microfluidic paper-based device for elisa. 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. pp. 75-78 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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abstract = "This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.",
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Liu, XY, Cheng, CM, Martinez, AW, Mirica, KA, Li, XJ, Phillips, ST, Mascareñas, M & Whitesides, GM 2011, A portable microfluidic paper-based device for elisa. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734365, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 75-78, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 1/23/11. https://doi.org/10.1109/MEMSYS.2011.5734365

A portable microfluidic paper-based device for elisa. / Liu, X. Y.; Cheng, C. M.; Martinez, A. W.; Mirica, K. A.; Li, X. J.; Phillips, S. T.; Mascareñas, M.; Whitesides, G. M.

2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 75-78 5734365 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

AB - This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

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Liu XY, Cheng CM, Martinez AW, Mirica KA, Li XJ, Phillips ST et al. A portable microfluidic paper-based device for elisa. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 75-78. 5734365. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734365