@inbook{b3817114cf704584b06181674d030f24,
title = "A practical guide to the use of monolayer purification and affinity grids",
abstract = "Lipid monolayers have traditionally been used in electron microscopy (EM) to form two-dimensional (2D) protein arrays for structural studies by electron crystallography. More recently, monolayers containing Nickel-nitrilotriacetic acid (Ni-NTA) lipids have been used to combine the purification and preparation of single-particle EM specimens of His-tagged proteins into a single, convenient step. This monolayer purification technique was further simplified by introducing the Affinity Grid, an EM grid that features a predeposited Ni-NTA lipid-containing monolayer. In this contribution, we provide a detailed description for the use of monolayer purification and Affinity Grids, discuss their advantages and limitations, and present examples to illustrate specific applications of the methods.",
author = "Kelly, {Deborah F.} and Danijela Dukovski and Thomas Walz",
year = "2010",
doi = "10.1016/S0076-6879(10)81004-3",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
number = "C",
pages = "83--107",
booktitle = "Methods in Enzymology",
address = "United States",
edition = "C",
}