A protective and broadly cross-neutralizing epitope of human papillomavirus L2

Ratish Gambhira, Balasubramanyam Karanam, Subhashini Jagu, Jeffrey N. Roberts, Christopher B. Buck, Ioannis Bossis, Hannah Alphs, Timothy Culp, Neil Christensen, Richard B.S. Roden

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.

Original languageEnglish (US)
Pages (from-to)13927-13931
Number of pages5
JournalJournal of virology
Volume81
Issue number24
DOIs
StatePublished - Dec 1 2007

Fingerprint

Human papillomavirus 16
Papillomaviridae
neutralization
epitopes
Epitopes
Bovine papillomavirus 1
Epidermodysplasia Verruciformis
peptides
Pseudoviridae
Condylomata Acuminata
Peptides
Subunit Vaccines
Passive Immunization
Human papillomavirus 18
Neutralizing Antibodies
Uterine Cervical Neoplasms
Virion
Immune Sera
warts
uterine cervical neoplasms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Gambhira, R., Karanam, B., Jagu, S., Roberts, J. N., Buck, C. B., Bossis, I., ... Roden, R. B. S. (2007). A protective and broadly cross-neutralizing epitope of human papillomavirus L2. Journal of virology, 81(24), 13927-13931. https://doi.org/10.1128/JVI.00936-07
Gambhira, Ratish ; Karanam, Balasubramanyam ; Jagu, Subhashini ; Roberts, Jeffrey N. ; Buck, Christopher B. ; Bossis, Ioannis ; Alphs, Hannah ; Culp, Timothy ; Christensen, Neil ; Roden, Richard B.S. / A protective and broadly cross-neutralizing epitope of human papillomavirus L2. In: Journal of virology. 2007 ; Vol. 81, No. 24. pp. 13927-13931.
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Gambhira, R, Karanam, B, Jagu, S, Roberts, JN, Buck, CB, Bossis, I, Alphs, H, Culp, T, Christensen, N & Roden, RBS 2007, 'A protective and broadly cross-neutralizing epitope of human papillomavirus L2', Journal of virology, vol. 81, no. 24, pp. 13927-13931. https://doi.org/10.1128/JVI.00936-07

A protective and broadly cross-neutralizing epitope of human papillomavirus L2. / Gambhira, Ratish; Karanam, Balasubramanyam; Jagu, Subhashini; Roberts, Jeffrey N.; Buck, Christopher B.; Bossis, Ioannis; Alphs, Hannah; Culp, Timothy; Christensen, Neil; Roden, Richard B.S.

In: Journal of virology, Vol. 81, No. 24, 01.12.2007, p. 13927-13931.

Research output: Contribution to journalArticle

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AU - Bossis, Ioannis

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AU - Culp, Timothy

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Gambhira R, Karanam B, Jagu S, Roberts JN, Buck CB, Bossis I et al. A protective and broadly cross-neutralizing epitope of human papillomavirus L2. Journal of virology. 2007 Dec 1;81(24):13927-13931. https://doi.org/10.1128/JVI.00936-07