A qualitative and quantitative analysis of proteins found in vespid venoms

Lorraine M. Mulfinger, Allen W. Benton, Miles W. Guralnick, Richard A. Wilson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Diagnosis and immunotherapy with venom extracts of patients sensitive to Hymenoptera stings has led to the problem of improving the standardization of Hymenoptera venom products. Current methods of standardization use the Lowry protein determination and a radial-diffusion assay for hyaluronidase activity. This study demonstrates that the results of these analyses do not always correlate with the actual quantity of allergenic protein present in the extracts. A method of standardization is examined herein that uses polyacrylamide gel electrophoresis to quantitate phospholipase A, antigen 5, and hyaluronidase, the proteins that together comprise most allergenic protein present in the venoms.' Also discussed in this study is the biochemical variability of phospholipase A and antigen 5 in the venoms of the different vespid species examined.

Original languageEnglish (US)
Pages (from-to)681-686
Number of pages6
JournalThe Journal of Allergy and Clinical Immunology
Volume77
Issue number5
DOIs
StatePublished - May 1986

Fingerprint

Wasp Venoms
Venoms
Hymenoptera
Hyaluronoglucosaminidase
Phospholipases A
Proteins
Antigens
Bites and Stings
Immunotherapy
Polyacrylamide Gel Electrophoresis

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Mulfinger, Lorraine M. ; Benton, Allen W. ; Guralnick, Miles W. ; Wilson, Richard A. / A qualitative and quantitative analysis of proteins found in vespid venoms. In: The Journal of Allergy and Clinical Immunology. 1986 ; Vol. 77, No. 5. pp. 681-686.
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A qualitative and quantitative analysis of proteins found in vespid venoms. / Mulfinger, Lorraine M.; Benton, Allen W.; Guralnick, Miles W.; Wilson, Richard A.

In: The Journal of Allergy and Clinical Immunology, Vol. 77, No. 5, 05.1986, p. 681-686.

Research output: Contribution to journalArticle

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