TY - JOUR
T1 - A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish
AU - Scheer, Nico
AU - Riedl, Iris
AU - Warren, J. T.
AU - Kuwada, John Y.
AU - Campos-Ortega, José A.
N1 - Funding Information:
We are grateful to Christel Schenkel for expert technical assistance and our colleagues for discussions. This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG, SFB 243, SFB 572) and the Fonds der Chemischen Industrie to J.A.C.-O. and by a NINDS grant (NS36587) to J.Y.K.
PY - 2002
Y1 - 2002
N2 - Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5h after heat shock (HS), persisted for at least 13h. myc-notch1a-intra RNA is expressed approximately 1.5h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.
AB - Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5h after heat shock (HS), persisted for at least 13h. myc-notch1a-intra RNA is expressed approximately 1.5h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.
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U2 - 10.1016/S0925-4773(01)00621-9
DO - 10.1016/S0925-4773(01)00621-9
M3 - Article
C2 - 11850174
AN - SCOPUS:0036182098
VL - 112
SP - 9
EP - 14
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -