A quantitative method for staging mouse embryos based on limb morphometry

Marco Musy, Kevin Flaherty, Jelena Raspopovic, Alexandre Robert-Moreno, Joan Therese Richtsmeier, James Sharpe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To determine the developmental stage of embryonic mice, we apply a geometric morphometric approach to the changing shape of the mouse limb bud as it grows from embryonic day 10 to embryonic day 15 post-conception. As the ontogenetic sequence results in the de novo emergence of shape features not present in the early stages, we have created a standard ontogenetic trajectory for limb bud development – a quantitative characterization of shape change during limb morphogenesis. This trajectory of form as a function of time also gives us the reverse function: the ability to infer developmental stage from form, with a typical uncertainty of 2 h. We introduce eMOSS (embryonic mouse ontogenetic staging system) as a fast, reliable, convenient and freely available online tool for staging embryos from two-dimensional images of their limb buds, and illustrate its use in phenotyping early limb abnormalities.

Original languageEnglish (US)
Article numberdev154856
JournalDevelopment (Cambridge)
Volume145
Issue number7
DOIs
StatePublished - Apr 1 2018

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Limb Buds
Embryonic Structures
Extremities
Morphogenesis
Uncertainty

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Musy, M., Flaherty, K., Raspopovic, J., Robert-Moreno, A., Richtsmeier, J. T., & Sharpe, J. (2018). A quantitative method for staging mouse embryos based on limb morphometry. Development (Cambridge), 145(7), [dev154856]. https://doi.org/10.1242/dev.154856
Musy, Marco ; Flaherty, Kevin ; Raspopovic, Jelena ; Robert-Moreno, Alexandre ; Richtsmeier, Joan Therese ; Sharpe, James. / A quantitative method for staging mouse embryos based on limb morphometry. In: Development (Cambridge). 2018 ; Vol. 145, No. 7.
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Musy, M, Flaherty, K, Raspopovic, J, Robert-Moreno, A, Richtsmeier, JT & Sharpe, J 2018, 'A quantitative method for staging mouse embryos based on limb morphometry', Development (Cambridge), vol. 145, no. 7, dev154856. https://doi.org/10.1242/dev.154856

A quantitative method for staging mouse embryos based on limb morphometry. / Musy, Marco; Flaherty, Kevin; Raspopovic, Jelena; Robert-Moreno, Alexandre; Richtsmeier, Joan Therese; Sharpe, James.

In: Development (Cambridge), Vol. 145, No. 7, dev154856, 01.04.2018.

Research output: Contribution to journalArticle

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