We describe a highly specific RIA for blood and urine 6-keto-PGF1 alpha, the principal metabolite of PGI2. The method requires extraction and column chromatography prior to assay and uses a tritium trace for assay monitoring. Dextran-coated charcoal is used to separate bound from free trace. Acceptable standard curve reproducibility over six assays had a mean correlation of 0.991 with a coefficient of variation of 6.61%. Good precision and accuracy data were generated with this assay, and normal human blood levels in 80 control subjects was less than 125 pg/ml. Significant elevations of blood 6-keto-PGF1 alpha were found in a high proportion of cancer patients with various solid tumor malignancies.
|Original language||English (US)|
|Number of pages||7|
|Journal||Advances in prostaglandin and thromboxane research|
|State||Published - Jan 1 1980|
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