A rapid and sensitive method for the detection of histone peptides

Dennis J. O'Kane, Robert Levenson, Raymond F. Jones

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H2A, H2B, and H4) prepared from oocytes of Xenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalAnalytical Biochemistry
Volume86
Issue number1
DOIs
StatePublished - Jan 1 1978

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A rapid and sensitive method for the detection of histone peptides'. Together they form a unique fingerprint.

  • Cite this