A rapid method for extraction of total lipids from whey protein concentrates and separation of lipid classes with solid phase extraction

M. N. Vaghela, A. Kilara

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

A modified procedure for extraction of total lipids from whey protein concentrates was developed such that stable emulsion with extracting solvents was avoided and the solvent system remained monophasic. Nonlipid contaminants from the extract were removed using gel filtration instead of traditional aqueous washing to prevent any loss of polar lipids. The extraction of total lipids by the modified procedure was complete and comparable with a reference procedure. Traditional thin-layer chromatography is tedious and more qualitative than quantitative for lipid class separation. Total lipids were further separated into free fatty acids, phospholipids, cholesterol ester, triacylglycerol, cholesterol, diacylglycerol, and monoacylglycerol, using modified solid phase extraction procedure. Columns with 2 g amino propyl packing allowed separation of up to 80 mg of total lipids into lipid classes gravimetrically. The values for anhydrous milk fat for all lipid classes agreed with those in the literature. Separation of total lipids into lipid classes with solid phase extraction is easy, quantitative, and can also be performed on a preparative scale.

Original languageEnglish (US)
Pages (from-to)1117-1121
Number of pages5
JournalJournal of the American Oil Chemists' Society
Volume72
Issue number10
DOIs
StatePublished - Oct 1 1995

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Organic Chemistry

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