A rapid method for isolating glandular trichomes

Ellen H. Verger, Richard A. Grazzini, David Hesk, Diana Lynn Cox-foster, Richard Craig, Ralph O. Mumma

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. The technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. This method was applied to several plants that bear similar stalked trichomes: geranium (Pelargonium), potato (Solanum tuberosum), tomato (Lycopersicon esculentum), squash (Cucurbita pepo), and velvetleaf (Abutilon theophrasti). The tissue preparation was of sufficient quality without further purification for biochemical and molecular studies. The preparation maintained the biochemical integrity of the trichomes for active enzymes and usable nucleic acids. A large quantity of tissue can be harvested; for example, 351 milligrams dry weight of glandular trichomes were harvested from geranium pedicels in 12 hours. The utility of the technique was demonstrated by examining the fatty acid composition of tall glandular trichomes of geraniums, Pelargonium xnortorum L.H. Bailey. These purified cells contained high concentrations of unusual ω5-unsaturated fatty acids, proportionally 23.4% of total fatty acids in the trichomes. When the trichomes were removed, the supporting tissue contained no ω5-fatty acids, thereby unequivocally localizing ω5-fatty acids to the trichomes. Because ω5-fatty acids are unique precursors for the biosynthesis of ω5-anacardic acids, we conclude that anacardic acid synthesis must occur in the glandular trichomes.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalPlant physiology
Volume99
Issue number1
StatePublished - Dec 1 1992

Fingerprint

Trichomes
rapid methods
trichomes
Fatty Acids
Pelargonium
Geranium
fatty acids
Cucurbita
Abutilon theophrasti
Lycopersicon esculentum
Solanum tuberosum
Anacardic Acids
Dry Ice
Cucurbita pepo
sieving
acids
pedicel
squashes
methodology
Unsaturated Fatty Acids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Verger, E. H., Grazzini, R. A., Hesk, D., Cox-foster, D. L., Craig, R., & Mumma, R. O. (1992). A rapid method for isolating glandular trichomes. Plant physiology, 99(1), 1-7.
Verger, Ellen H. ; Grazzini, Richard A. ; Hesk, David ; Cox-foster, Diana Lynn ; Craig, Richard ; Mumma, Ralph O. / A rapid method for isolating glandular trichomes. In: Plant physiology. 1992 ; Vol. 99, No. 1. pp. 1-7.
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Verger, EH, Grazzini, RA, Hesk, D, Cox-foster, DL, Craig, R & Mumma, RO 1992, 'A rapid method for isolating glandular trichomes', Plant physiology, vol. 99, no. 1, pp. 1-7.

A rapid method for isolating glandular trichomes. / Verger, Ellen H.; Grazzini, Richard A.; Hesk, David; Cox-foster, Diana Lynn; Craig, Richard; Mumma, Ralph O.

In: Plant physiology, Vol. 99, No. 1, 01.12.1992, p. 1-7.

Research output: Contribution to journalArticle

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Verger EH, Grazzini RA, Hesk D, Cox-foster DL, Craig R, Mumma RO. A rapid method for isolating glandular trichomes. Plant physiology. 1992 Dec 1;99(1):1-7.