A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

S. J. Wassner, J. L. Schlitzer, J. B. Li

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

Original languageEnglish (US)
Pages (from-to)284-289
Number of pages6
JournalAnalytical Biochemistry
Volume104
Issue number2
DOIs
StatePublished - May 15 1980

Fingerprint

High pressure liquid chromatography
High Pressure Liquid Chromatography
Urine
Ninhydrin
Plasmas
Ion Exchange Chromatography
Chromatography
Ion exchange
Healthy Volunteers
Pressure
Liquids
3-methylhistidine
sodium phosphate
acetonitrile

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{0e6aba22f7764fbebcc783d1cea3c8d9,
title = "A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography",
abstract = "A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.",
author = "Wassner, {S. J.} and Schlitzer, {J. L.} and Li, {J. B.}",
year = "1980",
month = "5",
day = "15",
doi = "10.1016/0003-2697(80)90076-7",
language = "English (US)",
volume = "104",
pages = "284--289",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography. / Wassner, S. J.; Schlitzer, J. L.; Li, J. B.

In: Analytical Biochemistry, Vol. 104, No. 2, 15.05.1980, p. 284-289.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

AU - Wassner, S. J.

AU - Schlitzer, J. L.

AU - Li, J. B.

PY - 1980/5/15

Y1 - 1980/5/15

N2 - A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

AB - A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

UR - http://www.scopus.com/inward/record.url?scp=0019313444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019313444&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(80)90076-7

DO - 10.1016/0003-2697(80)90076-7

M3 - Article

C2 - 7446954

AN - SCOPUS:0019313444

VL - 104

SP - 284

EP - 289

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -