A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

S. J. Wassner; J. L. Schlitzer; J. B. Li

doi:10.1016/0003-2697(80)90076-7

A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

S. J. Wassner, J. L. Schlitzer, J. B. Li

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 10³ times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

Original language	English (US)
Pages (from-to)	284-289
Number of pages	6
Journal	Analytical Biochemistry
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90076-7
State	Published - May 15 1980

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(80)90076-7

Cite this

@article{0e6aba22f7764fbebcc783d1cea3c8d9,

title = "A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography",

abstract = "A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.",

author = "Wassner, {S. J.} and Schlitzer, {J. L.} and Li, {J. B.}",

note = "Funding Information: This study was supported in part by grants from the Muscular Dystrophy Association and the National Institute of Arthritis, Metabolism and Digestive Disease (AM 14061).",

year = "1980",

month = may,

day = "15",

doi = "10.1016/0003-2697(80)90076-7",

language = "English (US)",

volume = "104",

pages = "284--289",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

AU - Wassner, S. J.

AU - Schlitzer, J. L.

AU - Li, J. B.

N1 - Funding Information: This study was supported in part by grants from the Muscular Dystrophy Association and the National Institute of Arthritis, Metabolism and Digestive Disease (AM 14061).

PY - 1980/5/15

Y1 - 1980/5/15

N2 - A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

AB - A method is presented for the precolumn derivatization and subsequent high-pressure liquid chromatographic separation of 3-methylhistidine from urine and plasma. The solvent system is 10 mm sodium phosphate (pH 7.5) and acetonitrile. The elution can be performed isocratically and requires less than 10 min. Both fluorescent and ultraviolet detection may be utilized. This method is at least 103 times more sensitive than conventional ion-exchange chromatography using ninhydrin. 3-Methylhistidine determinations performed on plasma and urine samples from normal volunteers correlated well with published literature values.

UR - http://www.scopus.com/inward/record.url?scp=0019313444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019313444&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(80)90076-7

DO - 10.1016/0003-2697(80)90076-7

M3 - Article

C2 - 7446954

AN - SCOPUS:0019313444

SN - 0003-2697

VL - 104

SP - 284

EP - 289

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

A rapid, sensitive method for the determination of 3-methylhistidine levels in urine and plasma using high-pressure liquid chromatography

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this