A review of analytical platforms for accurate bile acid measurement

Mainak Dutta, Jingwei Cai, Wei Gui, Andrew David Patterson

Research output: Contribution to journalReview article

Abstract

Bile acids are acidic steroids which help in lipid absorption, act as signaling molecules, and are key intermediate molecules between host and gut microbial metabolism. Perturbations in the circulating bile acid pool can lead to dysregulated metabolic and immunological function which may be associated with liver and intestinal disease. Bile acids have chemically diverse structures and are present in a broad range of concentrations in a wide variety of samples with complex biological matrices. Advanced analytical methods are therefore required to identify and accurately quantify individual bile acids. Though enzymatic determination of total bile acid is most popular in clinical laboratories, these methods provide limited information about individual bile acids. Advanced analytical methods such as gas chromatography- and liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy are highly informative techniques which help in identification and quantification of individual bile acids in complex biological matrices. Here, we review the detection technologies currently used for bile acid identification and quantification. We further discuss the advantages and disadvantages of these analytical techniques with respect to sensitivity, specificity, robustness, and ease of use. [Figure not available: see fulltext.].

Original languageEnglish (US)
Pages (from-to)4541-4549
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number19
DOIs
StatePublished - Jul 19 2019

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Bile Acids and Salts
Clinical laboratories
Intestinal Diseases
Molecules
Liquid chromatography
Metabolism
Liquid Chromatography
Gas chromatography
Gas Chromatography
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Mass Spectrometry
Magnetic Resonance Spectroscopy
Steroids
Technology
Lipids
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Dutta, Mainak ; Cai, Jingwei ; Gui, Wei ; Patterson, Andrew David. / A review of analytical platforms for accurate bile acid measurement. In: Analytical and Bioanalytical Chemistry. 2019 ; Vol. 411, No. 19. pp. 4541-4549.
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A review of analytical platforms for accurate bile acid measurement. / Dutta, Mainak; Cai, Jingwei; Gui, Wei; Patterson, Andrew David.

In: Analytical and Bioanalytical Chemistry, Vol. 411, No. 19, 19.07.2019, p. 4541-4549.

Research output: Contribution to journalReview article

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T1 - A review of analytical platforms for accurate bile acid measurement

AU - Dutta, Mainak

AU - Cai, Jingwei

AU - Gui, Wei

AU - Patterson, Andrew David

PY - 2019/7/19

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N2 - Bile acids are acidic steroids which help in lipid absorption, act as signaling molecules, and are key intermediate molecules between host and gut microbial metabolism. Perturbations in the circulating bile acid pool can lead to dysregulated metabolic and immunological function which may be associated with liver and intestinal disease. Bile acids have chemically diverse structures and are present in a broad range of concentrations in a wide variety of samples with complex biological matrices. Advanced analytical methods are therefore required to identify and accurately quantify individual bile acids. Though enzymatic determination of total bile acid is most popular in clinical laboratories, these methods provide limited information about individual bile acids. Advanced analytical methods such as gas chromatography- and liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy are highly informative techniques which help in identification and quantification of individual bile acids in complex biological matrices. Here, we review the detection technologies currently used for bile acid identification and quantification. We further discuss the advantages and disadvantages of these analytical techniques with respect to sensitivity, specificity, robustness, and ease of use. [Figure not available: see fulltext.].

AB - Bile acids are acidic steroids which help in lipid absorption, act as signaling molecules, and are key intermediate molecules between host and gut microbial metabolism. Perturbations in the circulating bile acid pool can lead to dysregulated metabolic and immunological function which may be associated with liver and intestinal disease. Bile acids have chemically diverse structures and are present in a broad range of concentrations in a wide variety of samples with complex biological matrices. Advanced analytical methods are therefore required to identify and accurately quantify individual bile acids. Though enzymatic determination of total bile acid is most popular in clinical laboratories, these methods provide limited information about individual bile acids. Advanced analytical methods such as gas chromatography- and liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy are highly informative techniques which help in identification and quantification of individual bile acids in complex biological matrices. Here, we review the detection technologies currently used for bile acid identification and quantification. We further discuss the advantages and disadvantages of these analytical techniques with respect to sensitivity, specificity, robustness, and ease of use. [Figure not available: see fulltext.].

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