A role for a 70-kilodaton heat shock protein in lysosomal degradation of intracellular proteins

Hui Ling Chiang, Stanley R. Terlecky, Charles P. Plant, J. Fred Dice

Research output: Contribution to journalArticlepeer-review

638 Scopus citations

Abstract

A 73-kilodalton (kD) intracellular protein was found to bind to peptide regions that target intracellular proteins for lysosomal degradation in response to serum withdrawal. This protein cross-reacted with a monoclonal antibody raised to a member of the 70-kD heat shock protein (hsp70) family, and sequences of two internal peptides of the 73-kD protein confirm that it is a member of this family. In response to serum withdrawal, the intracellular concentration of the 73-kD protein increased severalfold. In the presence of adenosine 5′-triphosphate (ATP) and MgCl2, the 73-kD protein enhanced protein degradation in two different cell-free assays for lysosomal proteolysis.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalScience
Volume246
Issue number4928
DOIs
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • General

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