A role for a 70-kilodaton heat shock protein in lysosomal degradation of intracellular proteins

Hui-ling Chiang, Stanley R. Terlecky, Charles P. Plant, J. Fred Dice

Research output: Contribution to journalArticle

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Abstract

A 73-kilodalton (kD) intracellular protein was found to bind to peptide regions that target intracellular proteins for lysosomal degradation in response to serum withdrawal. This protein cross-reacted with a monoclonal antibody raised to a member of the 70-kD heat shock protein (hsp70) family, and sequences of two internal peptides of the 73-kD protein confirm that it is a member of this family. In response to serum withdrawal, the intracellular concentration of the 73-kD protein increased severalfold. In the presence of adenosine 5′-triphosphate (ATP) and MgCl2, the 73-kD protein enhanced protein degradation in two different cell-free assays for lysosomal proteolysis.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalScience
Volume246
Issue number4928
DOIs
StatePublished - Jan 1 1989

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HSP70 Heat-Shock Proteins
Proteolysis
Proteins
Peptides
Magnesium Chloride
Heat-Shock Proteins
Serum
Adenosine Triphosphate
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • General

Cite this

Chiang, Hui-ling ; Terlecky, Stanley R. ; Plant, Charles P. ; Dice, J. Fred. / A role for a 70-kilodaton heat shock protein in lysosomal degradation of intracellular proteins. In: Science. 1989 ; Vol. 246, No. 4928. pp. 382-385.
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A role for a 70-kilodaton heat shock protein in lysosomal degradation of intracellular proteins. / Chiang, Hui-ling; Terlecky, Stanley R.; Plant, Charles P.; Dice, J. Fred.

In: Science, Vol. 246, No. 4928, 01.01.1989, p. 382-385.

Research output: Contribution to journalArticle

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