A Scaffold Free 3D Bioprinted Cartilage Model for In Vitro Toxicology

Pallab Datta; Yang Wu; Yin Yu; Kazim K. Moncal; Ibrahim T. Ozbolat

doi:10.1007/978-1-0716-0611-7_15

A Scaffold Free 3D Bioprinted Cartilage Model for In Vitro Toxicology

Pallab Datta, Yang Wu, Yin Yu, Kazim K. Moncal, Ibrahim T. Ozbolat

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Bioprinting has emerged as a promising method for precise spatiotemporal patterning of biological materials such as living cells, genetic materials, and proteins, which are sensitive to any other fabrication techniques. Bioprinting allows the generation of tissue constructs and models that closely mimic the anatomical and physiological attributes of a chosen tissue. In vitro toxicology assays can greatly benefit from bioprinting as drugs can be screened with higher efficiencies in a significantly reduced period. This protocol describes a method for fabricating bioprinted cartilage constructs which can be used for in vitro toxicology studies employing a scalable “tissue strand” bioprinting modality.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	175-183
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-0611-7_15
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2147
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-0611-7_15

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title = "A Scaffold Free 3D Bioprinted Cartilage Model for In Vitro Toxicology",

abstract = "Bioprinting has emerged as a promising method for precise spatiotemporal patterning of biological materials such as living cells, genetic materials, and proteins, which are sensitive to any other fabrication techniques. Bioprinting allows the generation of tissue constructs and models that closely mimic the anatomical and physiological attributes of a chosen tissue. In vitro toxicology assays can greatly benefit from bioprinting as drugs can be screened with higher efficiencies in a significantly reduced period. This protocol describes a method for fabricating bioprinted cartilage constructs which can be used for in vitro toxicology studies employing a scalable “tissue strand” bioprinting modality.",

author = "Pallab Datta and Yang Wu and Yin Yu and Moncal, {Kazim K.} and Ozbolat, {Ibrahim T.}",

note = "Funding Information: This work has been supported by National Science Foundation Award # 1624515. Pallab Datta acknowledges the Department of Science and Technology, Government of India, INSPIRE Faculty Award. The author thanks Dr. Weijie Peng (Penn State University) for his fruitful insights and Dr. Adil Akkouch (The University of Iowa) for his assistance with SEM imaging. The authors also thank Dr. James A. Martin (The University of Iowa) for providing facilities with mechanical testing.",

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AU - Moncal, Kazim K.

AU - Ozbolat, Ibrahim T.

N1 - Funding Information: This work has been supported by National Science Foundation Award # 1624515. Pallab Datta acknowledges the Department of Science and Technology, Government of India, INSPIRE Faculty Award. The author thanks Dr. Weijie Peng (Penn State University) for his fruitful insights and Dr. Adil Akkouch (The University of Iowa) for his assistance with SEM imaging. The authors also thank Dr. James A. Martin (The University of Iowa) for providing facilities with mechanical testing.

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