A selection system for identifying accessible sites in target RNAs

Wei Hua Pan, Heidi F. Devlin, Colleen Kelley, Harriet C. Isom, Gary Clawson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Although ribozymes offer tremendous potential for posttranscriptionally controlling expression of targeted genes, their utility is often limited by the accessibility of the targeted regions within the RNA transcripts. Here we describe a method that identifies RNA regions that are accessible to oligonucleotides. Based on this selection protocol, we show that construction of hammerhead ribozymes targeted to the identified regions results in catalytic activities that are consistently and substantially greater than those of ribozymes designed on the basis of computer modeling. Identification of accessible sites should also be widely applicable to design of antisense oligonucleotides and DNAzymes.

Original languageEnglish (US)
Pages (from-to)610-621
Number of pages12
JournalRNA
Volume7
Issue number4
DOIs
StatePublished - May 20 2001

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Catalytic RNA
Catalytic DNA
RNA
Antisense Oligonucleotides
Oligonucleotides
Gene Expression
hammerhead ribozyme

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Pan, Wei Hua ; Devlin, Heidi F. ; Kelley, Colleen ; Isom, Harriet C. ; Clawson, Gary. / A selection system for identifying accessible sites in target RNAs. In: RNA. 2001 ; Vol. 7, No. 4. pp. 610-621.
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A selection system for identifying accessible sites in target RNAs. / Pan, Wei Hua; Devlin, Heidi F.; Kelley, Colleen; Isom, Harriet C.; Clawson, Gary.

In: RNA, Vol. 7, No. 4, 20.05.2001, p. 610-621.

Research output: Contribution to journalArticle

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