A selective pathway for degradation of cytosolic proteins by lysosomes.

J. F. Dice, S. R. Terlecky, Hui-ling Chiang, T. S. Olson, L. D. Isenman, S. R. Short-Russell, S. Freundlieb, L. J. Terlecky

Research output: Contribution to journalReview article

49 Citations (Scopus)

Abstract

A lysosomal pathway of proteolysis is selective for cellular proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent cultured cells that are deprived of serum growth factors and in certain tissues of fasted animals. We have reconstituted this lysosomal degradation pathway in vitro. Transport into lysosomes requires a KFERQ-like sequence in the substrate protein and uptake and/or degradation is stimulated by ATP. A member of the heat shock 70 kDa protein family, the 73 kDa constitutive heat shock protein, binds to KFERQ-like peptide regions within proteins and, in some as yet unidentified manner, facilitates transfer of the proteins into lysosomes. Several possible mechanisms of selective protein transport into lysosomes are discussed.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalSeminars in Cell Biology
Volume1
Issue number6
StatePublished - Jan 1 1990

Fingerprint

Lysosomes
Proteolysis
Proteins
Peptides
HSP70 Heat-Shock Proteins
Protein Transport
Heat-Shock Proteins
Cultured Cells
Intercellular Signaling Peptides and Proteins
Adenosine Triphosphate
Serum

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Dice, J. F., Terlecky, S. R., Chiang, H., Olson, T. S., Isenman, L. D., Short-Russell, S. R., ... Terlecky, L. J. (1990). A selective pathway for degradation of cytosolic proteins by lysosomes. Seminars in Cell Biology, 1(6), 449-455.
Dice, J. F. ; Terlecky, S. R. ; Chiang, Hui-ling ; Olson, T. S. ; Isenman, L. D. ; Short-Russell, S. R. ; Freundlieb, S. ; Terlecky, L. J. / A selective pathway for degradation of cytosolic proteins by lysosomes. In: Seminars in Cell Biology. 1990 ; Vol. 1, No. 6. pp. 449-455.
@article{b6f250a73346405f97c87f02a59b4d22,
title = "A selective pathway for degradation of cytosolic proteins by lysosomes.",
abstract = "A lysosomal pathway of proteolysis is selective for cellular proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent cultured cells that are deprived of serum growth factors and in certain tissues of fasted animals. We have reconstituted this lysosomal degradation pathway in vitro. Transport into lysosomes requires a KFERQ-like sequence in the substrate protein and uptake and/or degradation is stimulated by ATP. A member of the heat shock 70 kDa protein family, the 73 kDa constitutive heat shock protein, binds to KFERQ-like peptide regions within proteins and, in some as yet unidentified manner, facilitates transfer of the proteins into lysosomes. Several possible mechanisms of selective protein transport into lysosomes are discussed.",
author = "Dice, {J. F.} and Terlecky, {S. R.} and Hui-ling Chiang and Olson, {T. S.} and Isenman, {L. D.} and Short-Russell, {S. R.} and S. Freundlieb and Terlecky, {L. J.}",
year = "1990",
month = "1",
day = "1",
language = "English (US)",
volume = "1",
pages = "449--455",
journal = "Seminars in Cell and Developmental Biology",
issn = "1084-9521",
publisher = "Academic Press Inc.",
number = "6",

}

Dice, JF, Terlecky, SR, Chiang, H, Olson, TS, Isenman, LD, Short-Russell, SR, Freundlieb, S & Terlecky, LJ 1990, 'A selective pathway for degradation of cytosolic proteins by lysosomes.', Seminars in Cell Biology, vol. 1, no. 6, pp. 449-455.

A selective pathway for degradation of cytosolic proteins by lysosomes. / Dice, J. F.; Terlecky, S. R.; Chiang, Hui-ling; Olson, T. S.; Isenman, L. D.; Short-Russell, S. R.; Freundlieb, S.; Terlecky, L. J.

In: Seminars in Cell Biology, Vol. 1, No. 6, 01.01.1990, p. 449-455.

Research output: Contribution to journalReview article

TY - JOUR

T1 - A selective pathway for degradation of cytosolic proteins by lysosomes.

AU - Dice, J. F.

AU - Terlecky, S. R.

AU - Chiang, Hui-ling

AU - Olson, T. S.

AU - Isenman, L. D.

AU - Short-Russell, S. R.

AU - Freundlieb, S.

AU - Terlecky, L. J.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - A lysosomal pathway of proteolysis is selective for cellular proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent cultured cells that are deprived of serum growth factors and in certain tissues of fasted animals. We have reconstituted this lysosomal degradation pathway in vitro. Transport into lysosomes requires a KFERQ-like sequence in the substrate protein and uptake and/or degradation is stimulated by ATP. A member of the heat shock 70 kDa protein family, the 73 kDa constitutive heat shock protein, binds to KFERQ-like peptide regions within proteins and, in some as yet unidentified manner, facilitates transfer of the proteins into lysosomes. Several possible mechanisms of selective protein transport into lysosomes are discussed.

AB - A lysosomal pathway of proteolysis is selective for cellular proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent cultured cells that are deprived of serum growth factors and in certain tissues of fasted animals. We have reconstituted this lysosomal degradation pathway in vitro. Transport into lysosomes requires a KFERQ-like sequence in the substrate protein and uptake and/or degradation is stimulated by ATP. A member of the heat shock 70 kDa protein family, the 73 kDa constitutive heat shock protein, binds to KFERQ-like peptide regions within proteins and, in some as yet unidentified manner, facilitates transfer of the proteins into lysosomes. Several possible mechanisms of selective protein transport into lysosomes are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0025583539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025583539&partnerID=8YFLogxK

M3 - Review article

C2 - 2103896

AN - SCOPUS:0025583539

VL - 1

SP - 449

EP - 455

JO - Seminars in Cell and Developmental Biology

JF - Seminars in Cell and Developmental Biology

SN - 1084-9521

IS - 6

ER -

Dice JF, Terlecky SR, Chiang H, Olson TS, Isenman LD, Short-Russell SR et al. A selective pathway for degradation of cytosolic proteins by lysosomes. Seminars in Cell Biology. 1990 Jan 1;1(6):449-455.