A sensitive assay for taxol and other microtubule-stabilizing agents

Charles D. Smith, Susan L. Mooberry, Xinqun Zhang, Anna Marija Helt

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The ability of taxol to protect microtubules in cultured human ovarian carcinoma cells from drug- and cold-induced depolymerization was characterized as a functional assay for microtubule stabilizing agents. Treatment of the cells with concentrations of vinblastine or colchicine of 50 nM or greater, or incubation at 4°C resulted in complete depolymerization of cytoplasmic microtubules. Pretreatment with taxol for 3 h enabled the cells to maintain substantial numbers of microtubules following the application of vinblastine or colchicine. This protective effect was easily observed at 50 nM taxol, whereas taxol-induced microtubule bundling was observed only at concentrations of 500 nM or greater. Concentrations of taxol as low as 10 nM stabilized microtubules against cold-induced depolymerization. Therefore, protection of microtubules from drug- and cold-induced depolymerization provides a sensitive functional assay for taxol. These systems should be similarly effective in identifying novel compounds which stabilize microtubules.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalCancer Letters
Volume79
Issue number2
DOIs
StatePublished - May 16 1994

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Smith, C. D., Mooberry, S. L., Zhang, X., & Helt, A. M. (1994). A sensitive assay for taxol and other microtubule-stabilizing agents. Cancer Letters, 79(2), 213-219. https://doi.org/10.1016/0304-3835(94)90262-3