A sensitive method for the quantification of the mass of inositol phosphates using gas chromatography-mass spectrometry

S. D. DaTorre, P. B. Corr, M. H. Creer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A sensitive method to directly measure the mass of inositol phosphates from biologic samples is described. The procedure uses ammonium sulfate gradient elution anion exchange column chromatography to isolate inositol monophosphate, bisphosphate, trisphosphate, and tetrakisphosphate. The isolated fractions are dephosphorylated and subsequently desalted by a novel approach using solid barium hydroxide in a 1:1 stoichiometric ratio to the amount of ammonium sulfate present in the dephosphorylated sample. The myo-inositol derived from each inositol phosphate species was quantified by stable isotope dilution gas chromatography-mass spectrometry of the hexakis(trimethylsilyl) derivative using hexadeutero-myo-inositol as the internal standard. The applicability and sensitivity of this method are illustrated by measuring the mass of individual inositol phosphates in isolated adult canine cardiac myocytes.

Original languageEnglish (US)
Pages (from-to)1925-1934
Number of pages10
JournalJournal of Lipid Research
Volume31
Issue number10
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

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