A sensitive, radiometric assay for lysophosphatidylcholine

David J. Dobmeyer, Peter B. Corr, Michael Creer

Research output: Contribution to journalArticle

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Abstract

To facilitate investigation of the metabolism of lysophosphatidylcholine and choline lysoplasmalogen in small quantities of tissue, a method for the quantification of these phospholipid species that is capable of accurate and reproducible analysis in samples which contain less than 1 nmol of total choline lysophospholipid was developed. The procedure employs chloroform and methanol extraction of phospholipids from isolated tissue with subsequent separation of the choline lysophospholipid fraction by high-performance liquid chromatography. The choline lysophospholipids are then acetylated with [3H]acetic anhydride and the [3H]acetyl-lysophosphatidylcholine product is isolated by thin-layer chromatography and quantified by liquid scintillation counting. The choline lysophospholipid content in the sample is determined from a standard curve constructed from samples containing a known amount of synthetic lysophosphatidylcholine with correction for recovery based on the inclusion of [14C]lysophosphatidylcholine as an internal standard.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalAnalytical Biochemistry
Volume185
Issue number1
DOIs
StatePublished - Feb 15 1990

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Lysophosphatidylcholines
Lysophospholipids
Choline
Assays
Phospholipids
Tissue
Scintillation Counting
Thin layer chromatography
High performance liquid chromatography
Scintillation
Thin Layer Chromatography
Chloroform
Metabolism
Methanol
High Pressure Liquid Chromatography
Recovery
Liquids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dobmeyer, David J. ; Corr, Peter B. ; Creer, Michael. / A sensitive, radiometric assay for lysophosphatidylcholine. In: Analytical Biochemistry. 1990 ; Vol. 185, No. 1. pp. 36-43.
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A sensitive, radiometric assay for lysophosphatidylcholine. / Dobmeyer, David J.; Corr, Peter B.; Creer, Michael.

In: Analytical Biochemistry, Vol. 185, No. 1, 15.02.1990, p. 36-43.

Research output: Contribution to journalArticle

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