A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis

A. B. Orth, M. Rzhetskaya, E. J. Pell, M. Tien

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance- conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.

Original languageEnglish (US)
Pages (from-to)2341-2345
Number of pages5
JournalApplied and environmental microbiology
Volume61
Issue number6
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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