A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis

A. B. Orth, M. Rzhetskaya, E. J. Pell, M. Tien

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance- conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.

Original languageEnglish (US)
Pages (from-to)2341-2345
Number of pages5
JournalApplied and environmental microbiology
Volume61
Issue number6
StatePublished - Jan 1 1995

Fingerprint

Ustilago
Ustilago zeae
fungicide resistance
vinclozolin
Protein-Serine-Threonine Kinases
plant pathogenic fungi
fungicide
Open Reading Frames
open reading frames
Fungi
dicarboximide fungicides
fungus
protein
Genomic Library
gene
genomic libraries
Nucleic Acid Databases
homology
protein kinases
Protein Kinases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis. / Orth, A. B.; Rzhetskaya, M.; Pell, E. J.; Tien, M.

In: Applied and environmental microbiology, Vol. 61, No. 6, 01.01.1995, p. 2341-2345.

Research output: Contribution to journalArticle

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