A simple, rapid, and sensitive method for measuring protein concentration in subcellular membrane fractions prepared by sucrose density ultracentrifugation

Ian A. Simpson, Ole Sonne

Research output: Contribution to journalArticlepeer-review

114 Scopus citations

Abstract

The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Sucrose in concentrations up to 0.7 m interfere only moderately with the assay. A rapid and convenient method is, therefore, now available for multiple protein determinations following sucellular fractionation on sucrose gradients.

Original languageEnglish (US)
Pages (from-to)424-427
Number of pages4
JournalAnalytical Biochemistry
Volume119
Issue number2
DOIs
StatePublished - Jan 15 1982

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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