A simple technique for the preparation and storage of sucrose gradients

Dawn S. Luthe

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

A method for preparing multiple sucrose gradients by quickly freezing layers of sucrose has been developed. These gradients may be stored in the freezer indefinitely, and thawed from 8 to 24 h at 4°C before use. The middle region of the resulting sucrose gradients was linear. Thawing time and centrifugation had little effect on the shape of the gradient. The method is applicable for both small- and large-volume centrifuge tubes. Gradients prepared in the same batch were nearly identical.

Original languageEnglish (US)
Pages (from-to)230-232
Number of pages3
JournalAnalytical Biochemistry
Volume135
Issue number1
DOIs
StatePublished - Nov 1983

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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