A single cell biosensor for probing bladder cancer heterogeneity

Peter Torab, Yue Yan, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Heterogeneity is a common feature of complex biological systems. The heterogeneity across multiple levels collectively drives a variety of (patho)physiological processes. Conventional biosensing approaches for characterizing molecular and cellular heterogeneity, however, are often limited due to the requirement of a large number of cells. Furthermore, existing single cell analysis techniques often require physical isolation or lysis of cells to “snapshot” RNA and protein biomarkers in a small subset of cells. Features of the complex microenvironment, such as hierarchical organization and dynamic cellular processes, are inherently lost by studying cells in isolation, fixation, and lysis. To address this grand challenge in biomedical research, we are developing a nanobiosensing platform based on DNA nanostructures and metallic nanoparticles to enable dynamic single cell gene expression analysis in patient-derived samples. Along with tumor biomanufacturing and 3D imaging strategies, we apply the single cell nanobiosensing platform for probing collective invasion of bladder cancer. Our results reveal the functional implications of tumor heterogeneity in muscle invasive bladder cancer, which will guide the development of prognostic and therapeutic strategies in the future.

Original languageEnglish (US)
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages285-286
Number of pages2
ISBN (Electronic)9781510897571
StatePublished - Jan 1 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume1

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period11/11/1811/15/18

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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  • Cite this

    Torab, P., Yan, Y., & Wong, P. K. (2018). A single cell biosensor for probing bladder cancer heterogeneity. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 285-286). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 1). Chemical and Biological Microsystems Society.